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Autophagy and apoptosis induced by Chinese giant salamander (Andrias davidianus) iridovirus (CGSIV)

文献类型： 外文期刊

作者： Du, Jian 1 ; Wang, Liqing 1 ; Wang, Yuanxian 1 ; Shen, Jian 1 ; Pan, Chuanyin 1 ; Meng, Yan 2 ; Yang, Changming 3 ; Ji, H 1 ;

作者机构： 1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

3.Anim Husb & Vet Stn Chenggu Cty, Hanzhong 723200, Peoples R China

关键词： Chinese giant salamander (Andrias davidianus); Iridovirus (CGSIV); Autophagy; Apoptosis; Immune activity

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2016 年 195 卷

页码：

收录情况： SCI

摘要： The outbreak of Chinese Giant Salamander (Andrias davidianus, CGS) Iridovirus (CGSIV) caused massive death of CGSs. However, some CGSs with low level of CGSIV usually could survive. In our study, major capsid protein (MCP) DNA replicates of CGSIV in shedding skin were employed to assess the relative content of CGSIV in the living CGSs by qPCR. Furthermore, the examinations of autophagy and apoptosis in CGSs in vivo and in the primary renal cells in vitro were performed, respectively. The results showed that the relative contents of CGSIV in the shedding skin could reflect those in liver, spleen, and kidney of the CGS5. In these tissues of the CGSs with low-level replicates of CGSIV, there were not obviously macroscopic lesions. But the irregularly-shaped vesicles perhaps involving in autophagosome were observed by transmission electron microscopy (TEM). The LC3B protein displayed uneven distribution by Immunohistochemistry and the level mRNA of Atg5 was higher in these tissues than that in the tissues of healthy CGSs using qRT-PCR. Meanwhile, the apoptosis also appeared in these tissues by TUNEL staining and higher level mRNA of type I IFN were detected in these tissues using qRT-PCR. Further, both the expression level of LC3B II protein and Atg5 mRNA increased significantly at 2 h after the virus infected the primary renal cells from the health CGSs in vitro. In addition, apoptosis and type I IFN mRNA began to increase significantly at 4 h after the virus infected the renal cells. It was suggested that autophagy may be a pivotal role for survival of CGSIV in the CGSs during early infection and the rapid proliferation of CGSIV could be inhibited by innate immune response and apoptosis. (C) 2016 Elsevier B.V. All rights reserved.

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