Comparative Study on Physical-Chemical and Biological Characteristics of Grass Carp Reovirus From Different Genotypes
文献类型： 外文期刊
作者： Wang, Qing 1 ; Zeng, Weiwei 1 ; Yin, Liang 1 ; Wang, Yingying 1 ; Liu, Chun 1 ; Li, Yingying 1 ; Zheng, Shucheng 1 ; Shi, 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab Fishery Drug Dev, Key Lab Aquat Anim Immune Technol, Minist Agr,Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
期刊名称：JOURNAL OF THE WORLD AQUACULTURE SOCIETY （ 影响因子：2.512； 五年影响因子：2.47 ）
ISSN： 0893-8849
年卷期： 2016 年 47 卷 6 期
页码：
收录情况： SCI
摘要： Grass carp reovirus (GCRV) was first isolated in 1983. In China, the virus caused severe hemorrhagic disease with significant losses of fingerling and yearling grass carp, Cyenopharyngodon idellus. Clade analysis of the different GCRV isolates from China indicates there are three distinct branches, which represent genotypes I, II, and III. However, little is known about the physical-chemical and biological characteristics of viruses from these three genotypes. In this study, the morphologic characteristics of JX-0901 (genotype I), HZ08 (genotype II), and Hubei grass carp disease reovirus (genotype III) were determined using electron microscopy which revealed morphological similarities but viral particles of the isolates arrayed in different ways. A comparison of selected viruses was performed for physical and chemical properties, including stability under different pH conditions, treatment with ether and trypsin, and repeated freeze-thaw cycles. Three isolates showed similar resistance to treatment with ether or trypsin and were stable between pH 3 and pH 10. The viruses exhibited different proliferation curves in the Grass carp swimming bladder cell. No postinfection mortalities or histological lesions were observed in infected rare minnow. Furthermore, cross-protection assays revealed low cross protection between different genotype viruses. This study contributes to the body of knowledge concerning disease control and vaccine development for grass carp hemorrhagic disease.
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