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Transcriptome analysis reveals that insulin is an immunomodulatory hormone in common carp

文献类型： 外文期刊

作者： Zhou, Yi 1 ; Yu, Wenjuan 3 ; Zhong, Huan 2 ; Li, Jianlin 3 ; Li, Hongxia 3 ; He, Feng 3 ; Zhou, Jie 3 ; Tang, Yongkai 3 ; Yu 1 ;

作者机构： 1.Changsha Univ, Dept Biotechnol & Environm Sci, Changsha 410003, Hunan, Peoples R China

2.Guangxi Acad Fishery Sci, Guangxi Key Lab Aquat Genet Breeding & Hlth Aquac, Nanning 530021, Peoples R China

3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Lab Freshwater Fisheries & Germplasm Resources Ut, Minist Agr, 9 East Shanshui Rd, Wuxi 214081, Peoples R China

4.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

关键词： Common carp; NF-kappa B; Toll-like receptor; RNA-seq; Transcriptome

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2016 年 59 卷

页码：

收录情况： SCI

摘要： Common carp (Cyprinus carpio) is a widespread freshwater fish and economically important species in China and other East Asian countries. Recent studies suggest that insulin can alter the expression of immune genes and, thus, can be regarded as an immunomodulatory hormone. To understand the mechanism of the immune response to insulin, we performed a comparative RNA-seq transcriptome analysis using livers from common carp injected with insulin (5 bodyweight) or saline as a control. After filtering the low-quality reads and removing the adaptors, the clean raw reads were assembled into 60,421 unigenes with mean length of 746.81 bp. Furthermore, 37,107 unigenes were annotated based on homology after blast search in public databases. Differentially expressed genes were identified using the fragments per kb per million fragments method and EdgeR software. In total, 782 differentially expressed genes were found. Thereinto, 444 and 338 genes were upregulated and downregulated, respectively, in the insulin-injected group. A Gene Ontology analysis indicated that these genes were concentrated in glucose metabolism, hormone secretion, andimmune system processes. Moreover, 153 enriched KEGG pathways were associated with the differentially expressed genes, including the Toll-like receptor (TLR) and nuclear factor kappa beta (NF-kappa B) signaling pathways. Signal transducer and activator of transcription 1 (10.56-fold), TLR3 (0.089-fold), activator protein-1 (0.007-fold), tumor necrosis factor-alpha (0.139-fold), and macrophage inflammatory protein-10 (0.038-fold) expression were significantly changed after the insulin injection. This study characterized the profile of genes expression response to insulin in common carp liver for the first time and provided new insight into understanding the molecular mechanism of insulin as an immunomodulatory hormone. (C) 2016 Elsevier Ltd. All rights reserved.

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