Molecular diagnostic markers of Tachysurus fulvidraco and Leiocassis longirostris and their hybrids
文献类型： 外文期刊
作者： Liang, Hongwei 1 ; Guo, Shanshan 1 ; Luo, Xiangzhong 1 ; Li, Zhong 1 ; Zou, Guiwei 1 ;
作者机构： 1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, 8,1st Wudayuan Rd, Wuhan 430223, Peoples R China
2.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
关键词： Molecular diagnostic markers; COI; ITS; Tachysurus fulvidraco; Leiocassis longirostris
期刊名称：SPRINGERPLUS （ 影响因子：1.13； 五年影响因子：1.29 ）
ISSN： 2193-1801
年卷期： 2016 年 5 卷
页码：
收录情况： SCI
摘要： Background: Bagridae is an important family of catfishes and has a high market demand. Recently, more cultivable Bagridae fishes are being exploited in China, and hybridization of some species has been carried out to achieve better growth performance, favorable sex ratios and better disease resistance. Yet, these hybrids have further increased the difficulties of taxonomy identification due to morphological indistinguishableness. Results: In this study, the molecular identification technologies for Tachysurus fulvidraco, Leiocassis longirostris sand their hybrids were successfully established by using mitochondrial COI and nuclear ITS sequences to identify the maternal and paternal lineage, respectively. Conclusion: These molecular diagnostic methods could also be used to manage breeding plans of hybrids, monitor and minimize the negative impacts of hybridization programs in aquaculture. Furthermore, our study could also provide a reference for establishing detection technique for hybrids in other groups of fishes.
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