文献类型： 外文期刊
作者： Yu, Fan 1 ; Yu, Juhua 1 ; Zhou, Yi 2 ; Yan, Jinpeng 3 ; Fang, Yanhong 5 ; Wang, Wenjun 5 ; Yang, Zhong 5 ;
作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi, Peoples R China
2.Changsha Univ, Dept Biotechnol & Environm Sci, Changsha, Hunan, Peoples R China
3.Cent S Univ, State Key Lab Med Genet, Changsha, Hunan, Peoples R China
4.Cent S Univ, Sch Life Sci, Changsha, Hunan, Peoples R China
5.Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Minist Water Resources, Chuxiong Rd 578, Wuhan 430079, Peoples R China
6.Chinese Acad Sci, Chuxiong Rd 578, Wuhan 430079, Peoples R China
关键词： Ameiurus melas; GC-skew; mitochondrial DNA; phylogenetic analysis
期刊名称：MITOCHONDRIAL DNA PART A （ 影响因子：1.514； 五年影响因子：0.707 ）
ISSN： 2470-1394
年卷期： 2016 年 27 卷 6 期
页码：
收录情况： SCI
摘要： In our research, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of Ameiurus melas. The total length of the mitochondrial genome is 16 512 bp and deposited in the GenBank with accession no. KT804702. The gene arrangement and transcriptional direction were similar to other bony fishes which contained 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region. The G contents were lowest (17.36%) and the nucleotide skewness for the coding strands of A. melas (GC-skew = -0.24) is biased toward G and the negative GC-skew ranges from -0.44 (ND6) to -0.13 (CO1). The phylogenetic studies indicate that A. melas and the Ictalurus punctatus are cluster together, they are sister group. However, the phylogenetic relationship of other Cyprininae has some differences, such as Pangasianodon gigas and Silurus asotus, which need further research.
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