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Natural variation in a calreticulin gene causes reduced resistance to Ca2+ deficiency-induced tipburn in Chinese cabbage (Brassica rapa ssp. pekinensis)

文献类型： 外文期刊

作者： Su, Tongbing 1 ; Li, Peirong 1 ; Wang, Huiping 2 ; Wang, Weihong 1 ; Zhao, Xiuyun 1 ; Yu, Yangjun 1 ; Zhang, Deshuang; 1 ;

作者机构： 1.BAAFS, BVRC, Beijing 100097, Peoples R China

2.Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China

3.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China

4.Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China

关键词： calcium; calreticulin; genome-wide association mapping; quantitative trait locus

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.228； 五年影响因子：7.791 ）

ISSN： 0140-7791

年卷期： 2019 年 42 卷 11 期

页码：

收录情况： SCI

摘要： Tipburn is an irreversible physiological disorder of Chinese cabbage that decreases crop value. Because of a strong environmental component, tipburn-resistant cultivars are the only solution, although tipburn resistance genes are unknown in Chinese cabbage. We studied three populations of Chinese cabbage over four growing seasons under field conditions: (a) 194 diverse inbred lines, (b) a doubled haploid (DH100) population, and (c) an F-2 population. The 194 lines were genotyped using single nucleotide polymorphism markers, and genome-wide-association mapping showed that 24 gQTLs were significantly associated with tipburn disease index. Analysis of the DH100 and F-2 populations identified a shared tipburn-associated locus, gqbTRA06, that was found to cover the region defined by one of the 24 gQTLs. Of 35 genes predicted in the 0.14-Mb quantitative trait locus region, Bra018575 (calreticulin family protein, BrCRT2) showed higher expression levels during disease development. We cloned the two BrCRT2 alleles from tipburn-resistant (BrCRT2 (R)) and tipburn-susceptible (BrCRT2(S)) lines and identified a 51-bp deletion in BrCRT2(S). Overexpression of BrCRT2 (R) increased Ca2+ storage in the Arabidopsis crt2 mutant and also reduced cell death in leaf tips and margins under Ca2+-depleted conditions. Our results suggest that BrCRT2 is a possible candidate gene for controlling tipburn in Chinese cabbage.

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