文献类型： 外文期刊
作者： Zhao, Zhiyong 1 ; Zhang, Yanmei 1 ; Gong, Andong 2 ; Liu, Na 3 ; Chen, Shanshan 1 ; Zhao, Xiaoyan 1 ; Li, Xiaobei 1 ; Che 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Minist Agr, Lab Qual & Safety Risk Assessment Agroprod Shangh, Inst Agrofood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China
2.Xinyang Normal Univ, Coll Life & Sci, Xinyang 464000, Peoples R China
3.Univ Chinese Acad Sci, Chinese Acad Sci, CAS Key Lab Nutr Metab & Food Safety, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, Huairou, Peoples R China
关键词： Biodegradation; Fumonisin B1; Bacterial consortium; Enzymatic detoxification; Metabolites
期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）
ISSN： 0175-7598
年卷期： 2019 年 103 卷 17 期
页码：
收录情况： SCI
摘要： Fumonisin B1 (FB1) contamination in cereals and cereal products remains an important aspect of food safety because of its wide distribution and the potential health hazard. However, only a few microorganisms have been reported to effectively degrade FB1. In this present study, a bacterial consortium SAAS79 with highly FB1-degrading activity was isolated from the spent mushroom compost. The combination of antibiotic-driven selection and 16S rDNA sequencing identified the Pseudomonas genus as the key FB1-degrading member. The microbial consortium could degrade more than 90% of 10 mu g/mL FB1 after incubation for 24 h at pH of 5-7 and temperature of 28-35 degrees C. The enzymes from the intracellular space were proved to be responsible for FB1 degradation, which eliminated about 90% of 10 mu g/mL FB1 in 3 h. Besides, liquid chromatography time-of-flight mass spectrometry (LC-TOF/MS) analysis identified two degradation products of FB1, and their toxicity on the monkey kidney cells (MARC-145) was significantly lower (p < 0.05) compared with the parent FB1. Overall, the consortium SAAS79 and its crude enzymes may be a potential choice for the decontamination of FB1 in the feed and food industry. Also, the bacterial consortium provides a new source of genes for the development of enzymatic detoxification agent.
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