GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton
文献类型： 外文期刊
作者： He, Peng 1 ; Wu, Shuyin 1 ; Jiang, Yanli 2 ; Zhang, Lihua 1 ; Tang, Meiju 1 ; Xiao, Guanghui 3 ; Yu, Jianing 1 ;
作者机构： 1.Shaanxi Normal Univ, Coll Life Sci, Xian 710119, Shaanxi, Peoples R China
2.Shanxi Acad Agr Sci, Cotton Res Inst, Yucheng 044000, Peoples R China
3.Shaanxi Normal Univ, Coll Life Sci, Key Lab,Minist Educ Med Plant Resources & Nat Pha, Natl Engn Lab Resource Dev Endangered Crude Drugs, Xian 710119, Shaanxi, Peoples R China
关键词： Chloroplast; PPR; Leaf variegation; Cotton
期刊名称：BMC PLANT BIOLOGY （ 2020影响因子：4.215； 五年影响因子：4.96 ）
ISSN： 1471-2229
年卷期： 2019 年 19 卷 1 期
收录情况： SCI
摘要： Background The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. PPR proteins function in organelles to target specific transcripts and are involved in plant development and growth. However, the function of PPR proteins in cotton is still unknown. Results In this study, we characterized a PPR gene YELLOW-GREEN LEAF (GhYGL1d) that is required for cotton plastid development. The GhYGL1d gene has a DYW domain in C-terminal and is highly express in leaves, localized to the chloroplast fractions. GhYGL1d share high amino acid-sequence homology with AtECB2. In atecb2 mutant, overexpression of GhYGL1d rescued the seedling lethal phenotype and restored the editing of accD and ndhF transcripts. Silencing of GhYGL1d led to the reduction of chlorophyll and phenotypically yellow-green leaves in cotton. Compared with wild type, GhYGL1d-silenced cotton showed significant deformations of thylakoid structures. Furthermore, the transcription levels of plastid-encoded polymerase (PEP) and nuclear-encoded polymerase (NEP) dependent genes were decreased in GhYGL1d-silenced cotton. Conclusions Our data indicate that GhYGL1d not only contributes to the editing of accD and ndhF genes, but also affects the expression of NEP- and PEP-dependent genes to regulate the development of thylakoids, and therefore regulates leaf variegation in cotton.
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