Functions of a Glucan Synthase Gene GFGLS in Mycelia! Growth and Polysaccharide Production of Grifola frondosa
文献类型： 外文期刊
作者： Cui, Feng-Jie 1 ; Wu, Xi-Hong 1 ; Tao, Ting-Lei 1 ; Zan, Xin-Yi 1 ; Sun, Wen-Jing 1 ; Mu, Da-Shuai 3 ; Yang, Yan 4 ; Wu, 1 ;
作者机构： 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
2.Jiangxi Prov Engn & Technol Ctr Food Additives Bi, Dexing 334221, Peoples R China
3.Shandong Univ Weihai, Coll Marine Sci, Weihai 264209, Peoples R China
4.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China
关键词： Grifola frondosa; glucan synthase; polysaccharide; gene silencing; RNA interference; mycelial growth
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2019 年 67 卷 32 期
页码：
收录情况： SCI
摘要： Glucan synthase (GLS) gene is known to be involved in the fungal biosynthesis of cell wall, differentiation, and growth. In the present study, a glucan synthase gene (GFGLS) in the edible mushroom Grifola frondosa with a full sequence of 5927 bp encoding a total of 1781 amino acids was cloned and characterized for the first time. GFGLSp_is a membrane.protein_ containing two large transmembrane domains connected with a hydrophilic cytoplasmic domain. With a constructed dual promoter RNA silencing vector pAN7-gfgls-dual, a GFGLS-silencing transformant iGFGLS-3 had the lowest GFGLS transcriptional expression level (26.1%) with a shorter length and thinner appearance of the mycelia, as well as decreased mycelial biomass and exo-polysaccharide production of 5.02 and 0.38 g/L, respectively. Further analysis indicated that GFGLS silence influenced slightly the monosaccharide compositions and ratios of mycelial and exo-polysaccharide. These findings suggest that GFGLS could affect mycelial growth and polysaccharide production by downregulating the glucan synthesis.
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