Highly Specific Monoclonal Antibody and Sensitive Quantum Dot Beads-Based Fluorescence Immunochromatographic Test Strip for Tebuconazole Assay in Agricultural Products
文献类型： 外文期刊
作者： Wang, Yulong 1 ; Xu, Junli 1 ; Qiu, Yulou 3 ; Li, Pan 1 ; Liu, Beibei 1 ; Yang, Lifei 2 ; Barnych, Bogdan 5 ; Hammock, Br 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety,State Key Lab, Nanjing 210014, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
3.China Jiliang Univ, Zhejiang Proceincial Key Lab Biometrol & Inspect, Hangzhou 310018, Zhejiang, Peoples R China
4.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
5.Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA
6.Univ Calif Davis, UCD Comprehens Canc Ctr, Davis, CA 95616 USA
关键词： immunochromatographic test strip; monoclonal antibody; tebuconazole; fluorescence; quantum dot beads
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2019 年 67 卷 32 期
页码：
收录情况： SCI
摘要： A monoclonal antibody (mAb) was raised against tebuconazole (TEB) using a hapten where the p-chloro substituent of the TEB molecule was replaced with a long-chain carboxylic acid. The resulting mAb showed high sensitivity and specificity against TEB characterized by ELISA with a half-maximal inhibitory concentration (IC50) of 0.19 ng mL(-1) and with cross-reactivity (CR) values below 0.01% to several analogues of triazole fungicides. On the basis of the mAb produced, a quantum dot beads-based fluorescence immunochromatographic test strip assay (QBs-FITSA) was developed for rapid and sensitive detection of TEB in agricultural product samples. The QBs-FITSA exhibited a linear detection range from 0.02 to 1.25 ng mL(-1) with a limit of detection (LOD) of 0.02 ng mL(-1). Furthermore, using produced mAb, multiple high-throughput rapid immunoassay formats could be achieved as a convenient monitoring tool for evaluation of human and environmental exposure to TEB.
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