Maize Genetic Transformation by Agrobacterium tumefaciens Assisted with Emery and Sonication Treatment
文献类型： 外文期刊
作者： Liang, Xue-lian 1 ; Chu, Kun 1 ; Nie, Xiao-xiong 1 ; Feng, Hua-jie 1 ; Han, Gui-li 1 ; Yi, Wen-bin 1 ; Wu, Qi-jing 1 ; Su 1 ;
作者机构： 1.Zhongkai Univ Agr & Engineer, Coll Life Sch, Guangzhou 510225, Guangdong, Peoples R China
2.Shanxi Acad Agr Sci, Key Lab Crop Gene Resources & Germplasm Enhanceme, Minist Agr, Biotechnol Res Ctr, Taiyuan 030031, Shanxi, Peoples R China
关键词： Agrobacterium; Maize; Corundum powder; Ultrasonic; Germinated seeds; epsps gene
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）
ISSN： 1560-8530
年卷期： 2019 年 22 卷 4 期
页码：
收录情况： SCI
摘要： In the past three decades, Agrobacterium-mediated genetic transformation has been widely used in breeding and molecular biology research. However, the classical Agrobacterium transformation method must employ tissue culture procedures, which are considered tedious, lengthy, and expensive as well as being genotype dependent. In this study, the ultrasonication-assisted Agrobacterium treatment method was modified with the addition of emery to increase the treatment intensity to the apical meristem cells of germinating seeds. In this study, the feasibility of emery grits assisted Agrobacterium transformation for maize was preliminary evaluated by glyphosate screening and PCR detection, which suggested that epsps gene was introduced into the maize cultivar. The transformation rate was enhanced by the addition of the emery and sonication treatment. The method has the advantages of circumventing the tedious tissue culture procedures and being easy to apply. (C) 2019 Friends Science Publishers
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