Analyses of virus/viroid communities in nectarine trees by next-generation sequencing and insight into viral synergisms implication in host disease symptoms
文献类型： 外文期刊
作者： Xu, Yunxiao 1 ; Li, Shifang 1 ; Na, Chengyong 3 ; Yang, Lijuan 1 ; Lu, Meiguang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China
2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou, Hainan, Peoples R China
3.Agr Dev & Serv Ctr Wed, Inner Mongolia, Liaoning, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2019 年 9 卷
页码：
收录情况： SCI
摘要： We analyzed virus and viroid communities in five individual trees of two nectarine cultivars with different disease phenotypes using next-generation sequencing technology. Different viral communities were found in different cultivars and individual trees. A total of eight viruses and one viroid in five families were identified in a single tree. To our knowledge, this is the first report showing that the most-frequently identified viral and viroid species co-infect a single individual peach tree, and is also the first report of peach virus D infecting Prunus in China. Combining analyses of genetic variation and sRNA data for co-infecting viruses/viroid in individual trees revealed for the first time that viral synergisms involving a few virus genera in the Betaflexiviridae, Closteroviridae, and Luteoviridae families play a role in determining disease symptoms. Evolutionary analysis of one of the most dominant peach pathogens, peach latent mosaic viroid (PLMVd), shows that the PLMVd sequences recovered from symptomatic and asymptomatic nectarine leaves did not all cluster together, and intra-isolate divergent sequence variants co-infected individual trees. Our study provides insight into the role that mixed viral/viroid communities infecting nectarine play in host symptom development, and will be important in further studies of epidemiological features of host-pathogen interactions.
- 相关文献
作者其他论文 更多>>
-
First Report of Athelia rolfsii Causing Southern Blight on Basella alba in Malaysia
作者：Khoo, Ying Wei;Li, Shifang;Khoo, Ying Wei;Baadu, Rosina;Chong, Khim Phin;Tan, Hui Teng;Khaw, Yam Sim;Li, Shifang
关键词：Athelia rolfsii; Basella alba; fungi; Malaysia; pathogen detection; sclerotia; vegetables; yield loss and economic impacts
-
The Virome of Piper nigrum: Identification, Genomic Characterization, Prevalence, and Transmission of Three New Viruses of Black Pepper in China
作者：Ma, Yuxin;Che, Haiyan;Lin, Yating;Li, Shifang;Ma, Yuxin;Xing, Fei;Li, Shifang;Gao, Shengfeng
关键词：badnavirus; enamovirus; fabavirus; high-throughput sequencing; novel viruses; Piper nigrum; transmission; viral disease
-
Diverse Novel Viruses Coinfecting the Tropical Ornamental Plant Polyscias balfouriana in China
作者：Ma, Yuxin;Che, Haiyan;Lin, Yating;Li, Shifang;Ma, Yuxin;Gao, Shengfeng;Li, Shifang
关键词：Polyscias balfouriana; virus discovery; coinfection; ornamental plant; Betaflexiviridae; Closteroviridae; Capillovirus; Citrivirus; Crinivirus
-
Breeding of virus-resistant transgenic sugarcane by the integration of the Pac1 gene
作者：Wang, Wenzhi;Wang, Jungang;Feng, Xiaoyan;Shen, Llinbo;Feng, Cuilian;Zhao, Tingting;Zhang, Shuzhen;Wang, Wenzhi;Xiao, Hong;Li, Shifang
关键词：sugarcane; double-stranded RNA-specific ribonuclease; PAC1 gene; broad-spectrum virus-resistant; genetic engineering
-
In-silico primer designing and PCR for detection of monkeypox virus (MPXV)
作者：Khoo, Ying Wei;Li, Shifang;Li, Shifang;Khoo, Ying Wei;Chong, Khim Phin
关键词：Bioinformatics tools; Pandemic; Pathology; Zoonosis
-
Strawberry, a New Natural Host of Brassica Yellows Virus in China
作者：He, Chengyong;Zhao, Xiaoli;Fan, Lingjiao;Wang, Hongqing;He, Chengyong;Li, Shifang;Li, Shifang
关键词：Brassica yellows virus; strawberry; new natural host; first report
-
First Report of Cassytha filiformis parasitizing Justicia gendarussa in Malaysia
作者：Khoo, Ying Wei;Li, Shifang;Li, Shifang;Khoo, Ying Wei;Chong, Khim Phin
关键词：
