The Virome of Piper nigrum: Identification, Genomic Characterization, Prevalence, and Transmission of Three New Viruses of Black Pepper in China
文献类型： 外文期刊
作者： Ma, Yuxin 1 ; Xing, Fei 3 ; Che, Haiyan 1 ; Gao, Shengfeng 4 ; Lin, Yating 1 ; Li, Shifang 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China
2.Guizhou Univ, Minist Educ, Key Lab, State Key Lab Breeding Base Green Pesticide & Agr, Guiyang 550025, Peoples R China
3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
4.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning 571533, Peoples R China
关键词： badnavirus; enamovirus; fabavirus; high-throughput sequencing; novel viruses; Piper nigrum; transmission; viral disease
期刊名称：PLANT DISEASE （ 影响因子：4.614； 五年影响因子：5.33 ）
ISSN： 0191-2917
年卷期： 2022 年 106 卷 8 期
页码：
收录情况： SCI
摘要： Viral diseases are one of the main categories of diseases that cause substantial yield losses in black pepper. Disease symptoms in black pepper are generally complex and are often caused by both known and undescribed viruses. To identify and clarify the etiology of viral diseases in black pepper in Hainan, China, we conducted high-throughput sequencing (HTS) by targeting purified double-stranded RNA (dsRNA) and ribosomal RNA depleted total RNA (rRNA-depleted totRNA). Analysis of the data revealed the presence of one known virus, piper yellow mottle virus (PYMoV), and three newly identified viruses: black pepper virus F (BPVF) in the genus Fabavirus, black pepper virus E (BPVE) in the genus Enamovirus, and black pepper virus B (BPVB) in the genus Badnavirus. The dominant viruses in P. nigrum sampled in Hainan are PYMoV, with an incidence of 100%, followed by BPVF (84%, 133 of 158) and BPVB (66%, 105 of 158). Mechanical inoculation of sap extracts from source plants containing PYMoV, BPVF, and BPVB gave negative results on both herbaceous and woody host plants 60 days postinoculation (dpi). BPVF and PYMoV were successfully transmitted to virus-free seedlings of black pepper through bark grafting, while BPVB was experimentally undetectable up to 150 dpi. Seed transmission experiments showed that no target viruses were present in all 59 germinated seedlings. This study provides information on diagnosis, prevalence, and transmission of black-pepper-associated viruses.
- 相关文献
作者其他论文 更多>>
-
Viromics Reveals Two Novel Viruses of the Family Closteroviridae in Codiaeum variegatum Plant with Leaf Variegation Symptoms
作者：Che, Haiyan;Lin, Yating;Long, Haibo;Li, Ye;Li, Shifang;Ma, Yuxin;Gao, Shengfeng;Cai, Lulu;Li, Shifang
关键词：
Ampelovirus ;Closteroviridae ;Codiaeum variegatum ;Crinivirus ; leaf variegation; virome -
High-Throughput Sequencing-Based Detection and Characterisation of a Putative Emaravirus Infecting Scaevola taccada
作者：Che, Haiyan;Li, Min;Hu, Meijiao;Luo, Daquan;Long, Haibo;Che, Haiyan;Li, Min;Hu, Meijiao;Luo, Daquan;Long, Haibo
关键词：emaravirus; high-throughput sequencing;
Scaevola taccada -
RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants
作者：Zhang, Pengcheng;Wang, Leizhen;Hong, Yiguo;Zhang, Pengcheng;Wang, Leizhen;Hong, Yiguo;Zhang, Pengcheng;Tor, Mahmut;Hong, Yiguo;Zhang, Xinlian;Mohamed, Atef M.;Daros, Jose-Antonio;Li, Shifang
关键词：Chloroplast-replicative viroid;
DCLs ; ELVd; RNA silencing; vd-siRNA; Viroid-plant interactions -
Root Exudates from Areca catechu L. Intercropping System Promote Nutrient Uptake and Sustainable Production of Piper nigrum L.
作者：Li, Zhiyuan;Li, Zhiyuan;Zhao, Yaqi;Zu, Chao;Li, Zhigang;Zheng, Weiquan;Yu, Huan;Gao, Shengfeng;Liu, Shichao;Wang, Can;Yang, Jianfeng;Zhao, Yaqi;Zhang, Baogui;Zu, Chao;Li, Zhigang;Zheng, Weiquan;Yu, Huan;Gao, Shengfeng;Liu, Shichao;Wang, Can;Yang, Jianfeng;Zu, Chao;Li, Zhigang;Zheng, Weiquan;Yu, Huan;Gao, Shengfeng;Liu, Shichao;Wang, Can;Yang, Jianfeng;Wang, Xinxin
关键词：intercropping; root exudates; bacterial community; soil metabolites; mineral nutrients
-
Interaction Between Glycoside Hydrolase FsGH28c from Fusarium solani and PnPUB35 Confers Resistance in Piper nigrum
作者：Liu, Shichao;Liu, Ruibing;Gao, Shengfeng;Yang, Jianfeng;Tian, Tian;Sun, Shiwei;Liu, Shichao;Gao, Shengfeng;Yang, Jianfeng;Sun, Shiwei;Xing, Tianci;Zhang, Chong;Zhao, Chenchen
关键词：glycoside hydrolase;
Fusarium solani ; cell death -
Identification and molecular characterization of two novel viruses from Allamanda cathartica in China
作者：Che, Haiyan;Ma, Yuxin;Long, Haibo;Che, Haiyan;Long, Haibo;Wu, Yangxin
关键词：
-
As a Transitional Host, Weed Solanum nigrum L. Increases the Population Base of Root-Knot Nematode Meloidogyne enterolobii for the Next Season
作者：Pei, Yueling;Sun, Yanfang;Chen, Yuan;Feng, Tuizi;Che, Haiyan;Long, Haibo;Long, Haibo
关键词：Meloidogyne enterolobii; transitional host; Solanum nigrum L.; parasitic characteristics; population base
