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Porcine epidemic diarrhea virus ORF3 protein causes endoplasmic reticulum stress to facilitate autophagy

文献类型： 外文期刊

作者： Zou, Dehua 1 ; Xu, Jiaxin 1 ; Duan, Xulai 1 ; Xu, Xin 4 ; Li, Pengfei 5 ; Cheng, Lixin 1 ; Zheng, Liang 1 ; Li, Xingzhi 1 ;

作者机构： 1.HeiLongJiang BaYi Agr Univ, Coll Life Sci & Technol, Daqing 163319, Peoples R China

2.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150069, Heilongjiang, Peoples R China

3.HeiLongJiang BaYi Agr Univ, Biotechnol Ctr, Daqing 163319, Peoples R China

4.HeiLongJiang Acad Agr Sci, Branch Anim Husb & Vet, Qiqihar 161005, Peoples R China

5.Harbin Med Univ, Affiliated Hosp 5, Dept Nephrol, Daqing 163319, Peoples R China

关键词： Porcine epidemic diarrhea virus; Accessory protein ORF3; Endoplasmic reticulum stress; Apoptosis; Autophagy

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2019 年 235 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV), the causative agent of PED, is an enveloped, positive-stranded RNA virus in the genus Alphacoronavirus, family Coronaviridae, order Nidovirales. PEDV non-structural accessory protein ORF3 is an ion channel related to viral infectivity and pathogenicity. Our previous study showed that PEDV ORF3 has expression characteristic of aggregation in cytoplasm, but its biological function remains elusive. Thus in this study, we initiated the construction of various vectors to express ORF3, and found ORF3 localized in the cytoplasm in the aggregation manner. Subsequently, confocal microscopy analysis showed that the aggregated ORF3 localized in endoplasmic reticulum (ER) to trigger ER stress response via up-regulation of GRP78 protein expression and activation of PERK-eIF2 alpha signaling pathway. In addition, our results showed that PEDV ORF3 could induce the autophagy through inducing conversion of LC3-I to LC3-II, but couldn't influence the apoptosis. In contrast, conversion of LC3-I/LC3-II could be significantly inhibited by 4-PBA, an ER stress inhibitor, indicating that ORF3-induced autophagy is dependent on ER stress response. This work not only provides some new findings for the biological function of the PEDV ORF3 protein, but also help us for the further understanding the molecular interaction between PEDV ORF3 protein and cells.

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