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Transgenic tobacco expressing dsRNA of the arginine kinase gene exhibits enhanced resistance against Helicoverpa armigera

文献类型： 外文期刊

作者： Ai, Xin-yu 1 ; Ren, Suwei 1 ; Liu, Ning 2 ; Huang, Lina 1 ; Liu, Xiao-Ning 1 ;

作者机构： 1.Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830046, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Crop Variety Resources, Urumqi 830091, Peoples R China

关键词： Helicoverpa armigera; RNA interference; arginine kinase; transgenic tobacco; growth and development; antifeedant effect

期刊名称：BULLETIN OF INSECTOLOGY （ 影响因子：1.711； 五年影响因子：1.756 ）

ISSN： 1721-8861

年卷期： 2019 年 72 卷 1 期

页码：

收录情况： SCI

摘要： Helicoverpa armigera Hubner (cotton bollworm) affects commercial value crops and can cause major economic losses. The wide application of insecticides and Bacillus thuringiensis cotton (Bt-cotton) has led to the enhanced resistance of cotton bollworm along with insecticide pollution that affects food production and the environment. Transgenic plants that produce double-stranded RNA (dsRNA) to target insect genes are being developed as a pest control strategy. In this study, we used Agrobacterium-mediated transformation to produce transgenic tobacco plants expressing dsRNA to silence the H. armigera arginine kinase (H alpha AK) gene. Arginine kinase is a phosphotransferase that plays a critical role in cellular energy metabolism in invertebrates. Two different H alpha AK fragments, AK1 (containing the conserved domain) and AK2, were selected based on our previous experiment. When H. armigera second instar larvae were fed transgenic plants expressing H alpha AK1 and H alpha AK2 dsRNA for 2 days, H alpha AK transcript levels were significantly reduced by 86% and 74%, respectively. In addition, the ingestion of transgenic plants significantly delayed larval growth and development. An antifeedant bioassay showed that transgenic plant leaves had a significant antifeedant effect on H. armigera larvae based on the ecological interaction between the transgenic plants and the cotton bollworm. These results demonstrate the potential of plant-mediated RNAi for pest control.

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