De novo transcriptome assembly and co-expression network analysis of Cynanchum thesioides: Identification of genes involved in resistance to drought stress
文献类型： 外文期刊
作者： Zhang, Xiaoyan 1 ; Yang, Zhongren 1 ; Li, Zhi 2 ; Zhang, Fenglan 1 ; Hao, Lizhen 1 ;
作者机构： 1.Inner Mongolia Agr Univ, Coll Hort & Plant Protect, Inner Mongolia Key Lab Wild Peculiar Vegetable Ge, Hohhot 010019, Peoples R China
2.Inner Mongolia Acad Agr & Anim Husb Sci, Special Crops Inst, Hohhot 010031, Peoples R China
关键词： Cynanchum thesioides (Freyn) K. Schum.; Cynanchum thesioides (Freyn) K. Schum. var. australe (maxim.) Tsiang et P. T. Li.; Drought resistance; Illumina sequencing; Differentially expressed genes; Weighted gene co-expression network
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
ISSN： 0378-1119
年卷期： 2019 年 710 卷
页码：
收录情况： SCI
摘要： Cynanchum thesioides are upright, xerophytic shrubs that are widely distributed in arid and semi-arid areas of China, North Korea, Mongolia and Siberia. To date, little is known about the molecular mechanisms of drought resistance in C. thesioides. To better understand drought resistance, we used transcriptome analysis and Illumina sequencing technology on C. thesioides, to identify drought-responsive genes. Using de novo assembly 55,268 unigenes were identified from 207.58 Gb of clean data. Amongst these, 36,265 were annotated with gene descriptions, conserved domains, gene ontology terms and metabolic pathways. The sequencing results showed that genes that were differentially expressed (DEGs) under drought stress were enriched in pathways such as carbon metabolism, starch and sucrose metabolism, amino acid biosynthesis, phenylpropanoid biosynthesis and plant hormone signal transduction. Moreover, many functional genes were up-regulated under severe drought stress to enhance tolerance. Weighted gene co-expression network analysis showed that there were key hub genes related to drought stress. Hundreds of candidate genes were identified under severe drought stress, including transcriptional factors such as MYB, G2-like, ERF, C2H2, NAC, NF-X1, GRF, HD-ZIP, HB-other, HSF, C3H, GRAS, WRKY, bHLH and Trihelix. These data are a valuable resource for further investigation into the molecular mechanism for drought stress in C. thesioides and will facilitate exploration of drought resistance genes.
- 相关文献
作者其他论文 更多>>
-
Effects of Continuous Cropping and Application of Bio-organic Fertilizers on Growth, Yield and Quality of Sugar Beet under Reduced Chemical Fertilizer Application
作者：Guo, Xiaoxia;Song, Baiquan;Zhou, Jianchao;Guo, Xiaoxia;Tian, Lu;Li, Yinghao;Huang, Chunyan;Li, Zhi;Jian, Caiyuan;Han, Kang;Su, Wenbin;Zhang, Peng;Kong, Dejuan;Wang, Zhenzhen;Liu, Jia
关键词：Sugar beet; Continuous cropping; Bio-organic fertilizer; Growth; Yield; Sugar content
-
Construction of Gene Expression Profiles of the NAC Transcription Factor Family in Sugar Beet Under Abiotic Stress
作者：Sun, Yaqing;Li, Changlun;Li, Zhi;Jian, Caiyuan;Li, Ningning;Zhang, Shaoying;Li, Guolong;Li, Zhi;Jian, Caiyuan
关键词：Sugar beet; NAC transcription factor; Abiotic stress; Gene expression
-
Combined physiological and transcriptomic analyses to identify candidate genes involved in aging during storage of Allium mongolicum Regel. seeds
作者：Zhang, Xiaoyan;Guo, Fei;Huang, Xiumei;Zhang, Dong;Ning, Kezhen;Wei, Pengchao;Hao, Lizhen;Zhang, Fenglan;Yang, Zhongren;Li, Zhi;Yang, Zhongren
关键词：Allium mongolicum; Storage; Physiology; Transcriptome; Differentially expressed genes
-
Proteomic Analysis of the Effect of Accelerated Ageing on Allium mongolicum Seeds
作者：Song, Xiaoqing;Yang, Zhongren;Zhang, Dong;Zhang, Xiaoyan;Zhang, Fenglan;Liu, Jiecai;Song, Xiaoqing;Yang, Zhongren;Zhang, Dong;Zhang, Xiaoyan;Zhang, Fenglan;Liu, Jiecai;Yu, Chuanzong
关键词：accelerated ageing; Allium mongolicum; differential expression protein; proteomics; seed
-
Effects of drought stress on physiology and antioxidative activity in two varieties of Cynanchum thesioides
作者：Zhang, Xiaoyan;Yang, Zhongren;Zhang, Fenglan;Hao, Lizhen;Li, Zhi
关键词：Antioxidase system; Drought stress; Osmotic adjustment; Physiological response
-
Physiological responses and transcriptome analysis of the Kochia prostrata (L.) Schrad. to seedling drought stress
作者：Yang, Zhongren;Zhang, Fenglan;Shi, Fengling;Wang, Xiaojuan;Sun, Hailian;Qiu, Xiao;Yi, Fengyan;Yang, Ding;Wu, Jianghong
关键词：Kochia prostrate; drought stress; stomatal; transcription factors; physiological changes
-
Water and nitrogen supply on photosynthetic physiological response of sugar beet (Beta vulgaris) under mulched drip irrigation
作者：Li, Zhi;Li, Guolong;Sun, Yaqing;Zhang, Shaoying;Li, Zhi;Su, Wenbin;Fan, Fuyi;Huang, Chunyan;Guo, Xiaoxia;Jian, Caiyuan;Tian, Lu;Han, Kang;Ren, Xiaoyun;Gong, Qianheng
关键词：Irrigation volume; mulched drip irrigation; nitrogen application amount; photosynthetic performance; sugar beet
