A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting the pathogen of maize rough dwarf disease in China
文献类型： 外文期刊
作者： Du, Linlin 1 ; Shi, Wenjuan 1 ; Li, Xuejuan 1 ; Lan, Ying 2 ; Sun, Feng 2 ; Fan, Yongjian 2 ; Zhou, Tong 1 ; Zhou, Yijun 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China
3.Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Jiangsu, Peoples R China
4.Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Jiangsu, Peoples R China
5.Int Rice Res Inst, Nanjing 210095, Jiangsu, Peoples R China
6.Jiangsu Acad Agr Sci, Joint Lab, Nanjing 210095, Jiangsu, Peoples R China
关键词： MRDD; Rapid detection; RT-LAMP; Calcein; Diagnosis
期刊名称：AUSTRALASIAN PLANT PATHOLOGY （ 影响因子：1.599； 五年影响因子：1.708 ）
ISSN： 0815-3191
年卷期： 2019 年 48 卷 5 期
页码：
收录情况： SCI
摘要： Maize rough dwarf disease (MRDD) is one of the most serious viral diseases of maize in China. The object of this study is to develop a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid and sensitive detection of MRDD pathogen. Four RT-LAMP primers were designed on the basis of S10. Template RNA from infected leaves were used for RT-LAMP which were carried out under isothermal conditions at 61 degrees C for 90 min. RT-LAMP products were analyzed by electrophoresis in agarose gels or visual inspection of color change after staining with calcein. Sensitivity of the RT-LAMP assay was 100-fold higher than RT-PCR. This method can distinguish RBSDV from SRBSDV and the vectors carrying RBSDV from Rice stripe virus (RSV). The RT-LAMP assay shows excellent potential for application in the rapid and sensitive detection of MRDD pathogens in field samples or vector.
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