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Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant (Solanum melongena L.)

文献类型： 外文期刊

作者： Xiao, Xi-ou 1 ; Lin, Wenqiu 1 ; Li, Ke 1 ; Feng, Xuefeng 1 ; Jin, Hui 1 ; Zou, Huafeng 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, South Subtrop Crop Res Inst, Zhanjiang City 524091, Peoples R China

2.Zhanjiang City Key Lab Trop Crops Genet Improveme, Zhanjiang City 524091, Guangdong, Peoples R China

3.Room 507,Huxiu Rd 1, Zhanjiang 52491, Guangdong, Peoples R China

关键词： eggplant; ethyl methanesulfonate mutation; fruit color; gene function; whole-genome re-sequencing

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN：

年卷期： 2019 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Whole-genome sequences of four EMS (ethyl methanesulfonate)-induced eggplant mutants were analyzed to identify genome-wide mutations. In total, 173.01 GB of paired-end reads were obtained for four EMS-induced mutants and (WT) wild type and 1,076,010 SNPs (single nucleotide polymorphisms) and 183,421 indels were identified. The most common mutation type was C/G to T/A transitions followed by A/T to G/C transitions. The mean densities were one SNP per 1.3 to 2.6 Mb. The effect of mutations on gene function was annotated and only 7.2% were determined to be deleterious. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed 10 and 11 genes, which were nonsynonymous mutation or frameshift deletion in 48-5 and L6-5 involved in the anthocyanin biosynthesis or flavone and flavonol biosynthesis. QRT-PCR results showed that only the Sme2.5_06210.1_g00004.1, which was annotated as UFGT (Flavonoid galactosidase transferase), expression significantly decreased in the L6-5 mutant compared with the WT. Also, the Sme2.5_06210.1_g00004.1 expression was lower in the colorless eggplant compared with colorful eggplant in the natural eggplant cultivar. These results suggest that Sme2.5_06210.1_g00004.1 may play a key role in eggplant anthocyanin synthesis.

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