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Development of an enzyme-linked immunosorbent assay for camptothecin

文献类型： 外文期刊

作者： Yan, Liting 1 ; Nan, Xiang 2 ; Zhang, Cunzheng 3 ; Wang, Haifang 4 ; Huang, Xiaoyan 1 ; Hu, Jun 1 ; Liu, Yingqian 2 ;

作者机构： 1.Shaanxi Prov Peoples Hosp, Cent Lab, Res Ctr Cell Immunol Engn & Technol Shaanxi Prov, Xian 710068, Shaanxi, Peoples R China

2.Lanzhou Univ, Sch Pharm, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Food Qual & Safety Inspect, Nanjing 210014, Jiangsu, Peoples R China

4.Shaanxi Univ Tradit Chinese Med, Inst Integrated Med, Xian 712046, Shaanxi, Peoples R China

关键词： camptothecin; ELISA; hapten; monoclonal antibody

期刊名称：MOLECULAR MEDICINE REPORTS （ 影响因子：2.952； 五年影响因子：2.754 ）

ISSN： 1791-2997

年卷期： 2019 年 20 卷 2 期

页码：

收录情况： SCI

摘要： The use of camptothecin and its analogues has increased in clinical settings and in agriculture. Therefore, camptothecins and their derivatives, metabolites and degradation products are frequently found in the environment. Therefore, it is important to develop an ELI SA for the quantification of camptothecins in human plasma, plants, animal tissues and other matrices. The present study developed a novel competitive indirect ELI SA for camptothecin using a monoclonal antibody (MAb). In total, two haptens and various carrier proteins were tested to select the most suitable immunogen for the production of MAbs against camptothecin. Hapten 1 conjugated with keyhole limpet hemocyanin was selected for the preparation of MAb 5A3, and was used to establish a competitive indirect ELI SA for camptothecin. A total of three derivatives of camptothecin used in clinical practice were examined. Topotecan showed an IC50 value of 0.68 mu g/ml with a detection limit of 0.19 mu g/ml, belotecan showed an IC50 value of 0.87 mu g/ml with a detection limit of 0.22 mu g/ml and irinotecan showed an IC50 value of 2.85 mu g/ml with a detection limit of 0.47 mu g/ml. The cross-reactivity results suggested that the assay developed in the present study possessed a high sensitivity to camptothecin. Therefore, this immunoassay technique may be suitable for monitoring the levels of camptothecin in compound analysis, clinical applications, and analyses of food and environmental samples.

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