iTRAQ-based analysis of 17 beta-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis
文献类型： 外文期刊
作者： Zhu Ying 1 ; Li Yangzhen 1 ; Li Hailong 1 ; Wang Lei 1 ; Zhang Ning 1 ; Liu Yang 1 ; Meng Liang 1 ; Xu Xiwen 1 ; Dong Zhongd 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China
3.Qingdao Univ, Res Inst Metabd Dis, Qingdao 266003, Shandong, Peoples R China
关键词： Cynoglossus semilaevis; 17 beta-estradiol; sex reversal; proteome; iTRAQ analysis
期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）
ISSN： 2096-5508
年卷期： 2019 年 37 卷 5 期
页码：
收录情况： SCI
摘要： The phenomenon of sex dimorphism prevails among many fish species. It has attracted the general research interest due to its close relationship to fish growth and thus aquaculture productivity. In Chinese tongue sole (Cynoglossus semilaevis), 17 beta-estradiol (E2) can be used reportedly to induce the feminization of fish, although the detailed regulatory network remained elusive. We treated the tongue sole fry before sex differentiation with E2 (10 and 30 mu g/L) to identify potential targets of E2 in Chinese tongue sole. The E2 treatment indeed induced genetic male fish sex-reversal to phenotypic female. Using an iTRAQ-based comparative proteomic analysis, 409 proteins that differentially expressed after E2 induction were identified, including 259 up-regulated and 150 down-regulated proteins. Furthermore, 19 differentially expressed proteins identified in the comparative proteomic analysis were selected to assess their transcription and eight of them exhibited the same tendency. Functions of the eight proteins included mainly nucleotide and protein binding. Interestingly, a far upstream element-binding protein 3-like isoform exhibited the significant upregulation both at transcription and translation levels after E2 treatment. This work identified a set of candidate proteins for E2 response and deepened our understanding of E2 regulatory mechanism.
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