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Rhizobium sophorae, Rhizobium laguerreae, and two novel Rhizobium genospecies associated with Vicia sativa L. in Northwest China

文献类型： 外文期刊

作者： Zhang, Junjie 1 ; Shang, Yimin 1 ; Peng, Shanshan 1 ; Chen, Wenfeng 4 ; Wang, Entao 7 ; de Lajudie, Philippe 8 ; Li, B 1 ;

作者机构： 1.Zhengzhou Univ Light Ind, Coll Food & Bioengn, Zhengzhou 450000, Henan, Peoples R China

2.Henan Key Lab Cold Chain Food Qual & Safety Contr, Zhengzhou 450000, Henan, Peoples R China

3.Collaborat Innovat Ctr Food Prod & Safety Henan P, Zhengzhou 450002, Henan, Peoples R China

4.State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

5.China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China

6.China Agr Univ, Rhizobium Res Ctr, Beijing 100193, Peoples R China

7.Inst Politecn Nacl, Dept Microbiol, Escuela Nacl Ciencias Biol, Mexico City 11340, DF, Mexico

8.Univ Montpellier, IRD, LSTM, CIRAD,INRA,SupAgro, Campus Baillarguet TA-A-82-J, F-34398 Montpellier 5, France

9.Henan Acad Agr Sci, Inst Plant Nutr & Resource Environm, Zhengzhou 450000, Henan, Peoples R China

关键词： Vicia sativa; Rhizobium sophorae; Rhizobium laguerreae; Rhizobium spp; nov; null; Northwest China

期刊名称：PLANT AND SOIL （ 影响因子：4.192； 五年影响因子：4.712 ）

ISSN： 0032-079X

年卷期： 2019 年 442 卷 1-2 期

页码：

收录情况： SCI

摘要： Aims To identify the rhizobia nodulating Vicia sativa in Northwestern China and to estimate their geographic distribution. Methods Rhizobia trapped with V. sativa plants from soils at six sites in the northwest of China were classified into genotypes by PCR-based restriction fragment length polymorphism (RFLP) of 16S-23S rRNA intergenic spacer (IGS) and 16S rRNA genes, and phylogenetic analyses of housekeeping (16S rRNA, recA, atpD) and symbiotic genes were performed for the representative strains. Soil physicochemical characteristics were recorded and canonical correlation analysis was performed to examine the correlations between soil features and distribution of rhizobial genotypes. Results A total of 202 rhizobial isolates were discriminated by RFLP analyses into 15 IGS types and a single 16S rRNA type, which were identified as Rhizobium by 16S rRNA gene phylogeny and as four clusters by multilocus sequence analysis (MLSA). Cluster 1 covering 86 strains and 7 IGS types prevalent in all sites was identified as Rhizobium laguerreae; cluster 2 was R. sophorae with 18 strains in 2 IGS types found in the site Q-GD. Each of cluster 3 and cluster 4 contained three IGS types representing two novel Rhizobium genospecies specific to Gansu Province and Shanxi Province, respectively. Four nodC phylogenetic clades were defined among the isolates. Conclusions R. sophorae, R. laguerreae, and two novel Rhizobium genospecies with diverse symbiotic genotypes are associated with Vicia sativa L. in Northwest China. Their biogeographic patterns are mainly directed by soil pH and salinity. This is the first study on the diversity of rhizobia nodulating Vicia sativa in China.

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