Delivery of plasmid DNA to shrimp hemocytes by Infectious hypodermal and hematopoietic necrosis virus (IHHNV) nanoparticles expressed from a baculovirus insect cell system
文献类型： 外文期刊
作者： Zhu, Yan-Ping 1 ; Li, Chen 1 ; Wan, Xiao-Yuan 1 ; Yang, Qian 1 ; Xie, Guo Si 1 ; Huang, Jie 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Minist Agr, Key Lab Maricultural Organism Dis Control, Qingdao, Shandong, Peoples R China
2.Chinese Acad Fishery Sci, Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China
4.Binzhou Med Univ, Precis Med Res Ctr, Yantai 264003, Shandong, Peoples R China
关键词： Infectious hypodermal and hematopoietic necrosis virus; Capsid protein; Virus-like particles; Disassembly/reassembly; Plasmid DNA
期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）
ISSN： 0022-2011
年卷期： 2019 年 166 卷
页码：
收录情况： SCI
摘要： Virus-like particles (VLPs) are potential containers for delivery of therapeutic agents at the nanoscale. In this study, the capsid protein of Infectious hypodermal and hematopoietic necrosis virus (IHHNV) was expressed in a baculovirus insect cell system. The 37-kDa recombinant protein containing the hexahistidine residues (His Tag) at N-terminal was purified using immobilized metal affinity chromatography (IMAC) and assembled into VLPs with a diameter of 23 +/- 3 nm analyzed by transmission electron microscopy. We also verified that disassembly/reassembly of IHHNV-VLPs was controlled in the presence and absence of DTT. The efficiency of IHHNV-VLPs to encapsulate plasmid DNA was about 48.2%, and the VLPs encapsulating the pcDNA3.1(+)-EGFP plasmid DNA could recognize the primary shrimp hemocytes and deliver the loaded plasmid into cells by detection of expressed enhanced green fluorescent protein (EGFP). These results implied that the IHHNV-VLPs might be a good candidate for packaging and delivery of expressible plasmid DNA, and may produce an antiviral product in shrimp cells for gene therapy.
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