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Identification and characterization of DAZ family genes in Chinese soft-shell turtle (Pelodiscus sinensis)

文献类型： 外文期刊

作者： Liu, Xiaoli 1 ; Tang, Zhoukai 1 ; Zhang, Piaoyi 1 ; Zhu, Xinping 1 ; Chu, Zhangjie 2 ; Li, Wei 1 ; Xu, Hongyan 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Key Lab Aquat Genom,Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

2.Zhejiang Ocean Univ, Fishery Sch, Zhoushan, Peoples R China

关键词： boule; dazl; germ cells; oogenesis; Pelodiscus sinensis; spermatogenesis

期刊名称：JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION （ 影响因子：2.656； 五年影响因子：2.473 ）

ISSN： 1552-5007

年卷期：

页码：

收录情况： SCI

摘要： The DAZ family genes, including boule, dazl, and daz, play pivotal roles in germ cell development and differentiation during gametogenesis in organisms, which have been widely studied in mammals, reptiles, or fishes. Dazl was bisexual expressed in both mitotic and meiotic germ cells, daz was male premeiotic expressed, whereas boule exhibits largely in unisexual meiotic germ cells but bisexual expression in several fishes, however, there is lack of report on boule gene and the evolutionary conservation and divergence of dazl and boule in reptile. Here, both boule and dazl genes were characterized in Pelodiscus sinensis. The quantitative real-time polymerase chain reaction analysis showed that boule and dazl were abundantly expressed in adult ovary and testis but barely in somatic tissues, such as heart, brain, liver, spleen, and kidney. Moreover, through fluorescent in situ hybridization, bisexual and germline-specific expression profiles of boule and dazl messenger RNAs (mRNAs) were demonstrated. Boule mRNA exhibited a maximal meiotic expression in spermatocytes, and a relatively low, but distinct expression in oocytes at meiotic stages in P. sinensis, similar to the expression profile of human boule in ovary. However, dazl mRNA was richly distributed in male germ cells at almost all stages during spermatogenesis, and predominantly expressed in most of stages of oocytes including premeiotic and meiotic stages. These findings imply that boule and dazl would play distinct roles in the sexual differentiation of germ cells during turtle gametogenesis, and the major functions of daz family members involved in germ cell differentiation would be conserved across species including P. sinensis.

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