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Identification of responsive miRNAs involved in combination stresses of phosphate starvation and salt stress in soybean root

文献类型： 外文期刊

作者： Ning, Li-Hua 1 ; Du, Wen-Kai 4 ; Song, Hai-Na 4 ; Shao, Hong-Bo 1 ; Qia, Wei-Cong 1 ; Amr Sheteiwy, Mohamed Salah 1 ;

作者机构： 1.JAAS, Inst Agr Resources & Environm, Salt Soil Agr Ctr, Key Lab Agr Environm Lower Reaches Yangtze River, Nanjing 210014, Jiangsu, Peoples R China

2.Yancheng Teachers Univ, Jiangsu Synthet Innovat Ctr Coastal Bioagr, Jiangsu Key Lab Bioresources Saline Soils, Yancheng 224002, Peoples R China

3.Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao 266000, Shandong, Peoples R China

4.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

5.Mansoura Univ, Fac Agr, Dept Agron, Mansoura 35516, Egypt

关键词： Salt stress; Pi starvation; Combination stresses; miRNA; Soybean

期刊名称：ENVIRONMENTAL AND EXPERIMENTAL BOTANY （ 影响因子：5.545； 五年影响因子：5.99 ）

ISSN： 0098-8472

年卷期： 2019 年 167 卷

页码：

收录情况： SCI

摘要： Salt stress and phosphate (Pi) starvation severely affect plants growth and development. MicroRNAs involved in single stress of Pi starvation or salt stress have been reported in many plant species. However, the combination stresses of Pi starvation and salt stress responsive microRNAs and their target genes have not been reported. In the, study, we generated 7 small RNA libraries under 4 different treatments with three independent biological replicates: CK, salt stress (SR1, SR5), Pi starvation (LPR1, LPR5), and the double stresses of salt stress/Pi starvation (DR1, DR5) in soybean root by using Illumina HiSeqTM2000 sequencing technique. Total of 5285 miRNAs including 3616 conserved and 1669 novel miRNAs were identified. Among them, 21 miRNAs including gma-miR1691-3p, gma-miR5036, gma-miR862a, and gma-398a/b, were detected to respond to the single stress and the double stresses of Pi starvation and salt stress. The target genes for the identified miRNAs such as ethylene-responsive transcription factor 4, protein phosphatase 2C, and superoxide dismutase [Cu-Zn], were classified into different biological processes including metabolic process, signaling transduction. The expression patterns of several identified miRNAs were validated by RT-qPCR and the results of RT-qPCR were consistent with the result of IlluminaHiSeq. The Na+, K+, and PO43- contents under different treatments were detected in soybean roots and leaves. Our findings identified the differentially expressed miRNAs in response to the combination stresses of Pi starvation and salt stress, which providing the essential information for enhancing the resistance to salt stress and Pi starvation in soybean.

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