Combinatorial Avidity Selection of Mosaic Landscape Phages Targeted at Breast Cancer Cells-An Alternative Mechanism of Directed Molecular Evolution
文献类型： 外文期刊
作者： Petrenko, Valery A. 1 ; Gillespie, James W. 1 ; Xu, Hai 1 ; O'Dell, Tiffany 1 ; De Plano, Laura M. 1 ;
作者机构： 1.Auburn Univ, Coll Vet, Dept Pathobiol, Auburn, AL 36849 USA
2.Jiangsu Acad Agr Sci, Natl Vet Biol Med Engn Res Ctr, Nanjing 210014, Jiangsu, Peoples R China
3.Univ Messina, Dept Chem Sci Biol Pharmaceut & Environm, Viale F Stagno dAlcontres 31, I-98166 Messina, Italy
关键词： landscape phage display; directed molecular evolution; combinatorial selection; short linear motifs; domain motif interaction; breast cancer
期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）
ISSN：
年卷期： 2019 年 11 卷 9 期
页码：
收录情况： SCI
摘要： Low performance of actively targeted nanomedicines required revision of the traditional drug targeting paradigm and stimulated the development of novel phage-programmed, self-navigating drug delivery vehicles. In the proposed smart vehicles, targeting peptides, selected from phage libraries using traditional principles of affinity selection, are substituted for phage proteins discovered through combinatorial avidity selection. Here, we substantiate the potential of combinatorial avidity selection using landscape phage in the discovery of Short Linear Motifs (SLiMs) and their partner domains. We proved an algorithm for analysis of phage populations evolved through multistage screening of landscape phage libraries against the MDA-MB-231 breast cancer cell line. The suggested combinatorial avidity selection model proposes a multistage accumulation of Elementary Binding Units (EBU), or Core Motifs (CorMs), in landscape phage fusion peptides, serving as evolutionary initiators for formation of SLiMs. Combinatorial selection has the potential to harness directed molecular evolution to create novel smart materials with diverse novel, emergent properties.
- 相关文献
作者其他论文 更多>>
-
Comparative transcriptomics analysis of tolerant and sensitive genotypes reveals genes involved in the response to cold stress in bitter gourd (Momordica charantia L.)
作者：Ning, Yu;Liu, Zhiyang;Liu, Jing;Xu, Hai;Chen, Longzheng;Qi, Renjie;Xia, Pengfei;Yuan, Xihan
关键词：
-
Genome-wide analyses of the NAC transcription factor family to reveal the potential candidate genes responding to powdery mildew in balsam pear
作者：Ning, Yu;Liu, Jing;Song, Bo;Xu, Hai;Liu, Zhiyang;Chen, Longzheng
关键词：Balsam pear; NAC transcription factors; Identification; Expression analysis; Powdery mildew
-
Construction of a T7 phage display nanobody library for bio-panning and identification of chicken dendritic cell-specific binding nanobodies
作者：Xu, Hai;Li, Ling;Hong, Weiming;Li, Ruiting;Guo, Zijie;Xu, Hai;Govinden, Roshini;Chenia, Hafizah Y.;Xu, Hai;Deng, Bihua;Hou, Jibo;Deng, Bihua
关键词：
-
Evaluation of dendritic cell-targeting T7 phages as a vehicle to deliver avian influenza virus H5 DNA vaccine in SPF chickens
作者：Xu, Hai;Li, Ling;Li, Ruiting;Guo, Zijie;Lin, Mengzhou;Xu, Hai;Lu, Yu;Hou, Jibo;Deng, Bihua;Xu, Hai;Govinden, Roshini;Chenia, Hafizah Y. Y.;Lu, Yu;Hou, Jibo;Deng, Bihua;Xu, Hai;Li, Ruiting;Guo, Zijie
关键词：avian influenza; DNA vaccine; dendritic cell-targeting; T7 phage; SPF chicken
-
Biological Characterization and Evolution of Bacteriophage T7-Delta holin During the Serial Passage Process
作者：Xu, Hai;Hong, Weiming;Wang, Anping;Dong, Hongyan;Xu, Hai;Bao, Xi;Hou, Jibo;Deng, Bihua;Xu, Hai;Govinden, Roshini;Chenia, Hafizah Y. Y.;Wang, Kaimin;Deng, Bihua
关键词：T7 phage; holin; lysis; compensatory host; evolution
-
Development of a water-in-oil-in-water adjuvant for foot-and-mouth disease vaccine based on ginseng stem-leaf saponins as an immune booster
作者：Xu, Hai;Hong, Weiming;Guo, Changming;Xu, Hai;Niu, Yale;Liu, Weixin;Zuo, Xiaoxin;Bao, Xi;Lu, Yu;Deng, Bihua;Lu, Yu;Deng, Bihua
关键词：Adjuvant; Double emulsion; Ginseng stem-leaf saponins; Foot and mouth disease vaccine
-
Study of the Relationship between Leaf Color Formation and Anthocyanin Metabolism among Different Purple Pakchoi Lines
作者：Song, Bo;Chen, Song;Xu, Zhigang;Song, Bo;Xu, Hai;Chen, Longzheng;Fan, Xiaoxue;Jing, Zange
关键词：pakchoi; purple leaf; color formation; anthocyanin
