您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

De novo transcriptome analysis of differentially expressed genes in the liver of pampus argenteus under temperature stress

文献类型： 外文期刊

作者： Shi, Zhaohong 1 ; Liu, Lei 1 ; Wang, Lumin 1 ; Gao, Quanxin 1 ; Peng, Shiming 1 ; Yue, Yanfeng 1 ;

作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Minist Agr, Key Lab Marine & Estuarine Fisheries, Shanghai 200090, Peoples R China

关键词： De novo transcriptome analysis; Pampus argenteus; Temperature stress

期刊名称：INDIAN JOURNAL OF ANIMAL RESEARCH （ 影响因子：0.44； 五年影响因子：0.543 ）

ISSN： 0367-6722

年卷期： 2019 年 53 卷 9 期

页码：

收录情况： SCI

摘要： Pampus argenteus is an aquatic cold-blooded animal, and its growth and survival are greatly affected by ambient temperature. During aquaculture, a sudden change in water temperature may be caused by climate change or other human factors, resulting in acute temperature stress. In this study, de novo transcriptome sequencing technology was used to analyze changes in the gene expressions in the liver of P. argenteus under temperature stress to understand the mechanisms of temperature regulation in P. argenteus. The results showed that 72447398, 69534310, 63698204, 78876728 and 53969050 clean reads were obtained from four cDNA libraries (A: 27 degrees C (control group), B: 22 degrees C and 6 h, C: 32 degrees C and 6 h, D: 22 degrees C and 12 h, E: 32 degrees C and 12 h) of the P. argenteus by Illumina sequencing technology. In A\B, A\C, A\D and A\E pairwise comparison, 353, 431, 1303 and 343 differentially expressed genes (DEGs) were detected, respectively. Of these genes, 67 genes were identified among all the pairwise comparisons as the common DEGs. Four genes related to metabolic adaption to temperature were randomly selected to validate the DEGs results by real time PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated some vital genes and pathways associated with metabolism in response to temperature challenge. These results will help us to understand the molecular mechanism underlying temperature regulation in P. argenteus and provide a theoretical basis for the study of related molecular mechanisms in fish under temperature stress.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台