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Screening 89 Pesticides in Fishery Drugs by Ultrahigh Performance Liquid Chromatography Tandem Quadrupole-Orbitrap Mass Spectrometer

文献类型： 外文期刊

作者： Wang, Shou-Ying 1 ; Kong, Cong 1 ; Chen, Qing-Ping 1 ; Yu, Hui-Juan 1 ;

作者机构： 1.East China Sea Fisheries Res Inst, Minist Agr & Rural, Lab Qual & Safety Risk Assessment Aquat Prod Shan, Shanghai 200090, Peoples R China

2.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

3.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab East China Sea Fishery Resources Exploita, Shanghai 200090, Peoples R China

关键词： fishery drugs; high-resolution orbitrap mass spectrometry; pesticide; screening

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期： 2019 年 24 卷 18 期

页码：

收录情况： SCI

摘要： Multiclass screening of drugs with high resolution mass spectrometry is of great interest due to its high time-efficiency and excellent accuracy. A high-scale, fast screening method for pesticides in fishery drugs was established based on ultrahigh performance liquid chromatography tandem quadrupole-Orbitrap high-resolution mass spectrometer. The target compounds - were diluted in methanol and extracted by ultrasonic treatment, and the extracts were diluted with MeOH-water (1:1, v/v) and centrifuged to remove impurities. The chromatographic separation was performed on an Accucore aQ-MS column (100 mm x 2.1 mm, 2.6 mu m) with gradient elution using 0.1% formic acid in water (containing 5 mmol/L ammonium formate) and 0.1% formic acid in methanol (containing 5 mmol/L ammonium formate) in Full Scan/dd-MS2 (TopN) scan mode. A screening database, including mass spectrometric and chromatographic information, was established for identification of compounds. The screening detection limits of methods ranged between 1-500 mg/kg, the recoveries of real samples spiked with the concentration of 10 mg/kg and 100 mg/kg standard mixture ranged from 70% to 110% for more than sixty compounds, and the relative standard deviations (RSDs) were less than 20%. The application of this method showed that target pesticides were screened out in 10 samples out of 21 practical samples, in which the banned pesticide chlorpyrifos were detected in 3 out of the 10 samples.

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