The ilvGMEDA Operon Is Regulated by Transcription Attenuation in Vibrio alginolyticus ZJ-T
文献类型： 外文期刊
作者： Deng, Yiqin 1 ; Luo, Xing 3 ; Xie, Mei 1 ; Bouloc, Philippe 3 ; Chen, Chang 1 ; Jacq, Annick 3 ;
作者机构： 1.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr & Rural Affairs, Guangzhou, Guangdong, Peoples R China
3.Univ Paris Sud, Univ Paris Saclay, Inst Integrat Biol Cell, CNRS,CEA, Gif Sur Yvette, France
4.Chinese Acad Sci, South China Sea Inst Oceanol, Xisha Nansha Ocean Observat & Res Stn, Guangzhou, Guangdong, Peoples R China
关键词： Vibrio alginolyticus; acetolactate synthase (AHAS); branched-chain amino acids; ilvGMEDA operon; leader attenuator; transcription attenuation
期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）
ISSN： 0099-2240
年卷期： 2019 年 85 卷 19 期
页码：
收录情况： SCI
摘要： Bacteria synthesize amino acids according to their availability in the environment or, in the case of pathogens, within the host. We explored the regulation of the biosynthesis of branched-chain amino acids (BCAAs) (L-leucine, L-valine, and L-isoleucine) in Vibrio alginolyticus, a marine fish and shellfish pathogen and an emerging opportunistic human pathogen. In this species, the ilvGMEDA operon encodes the main pathway for biosynthesis of BCAAs. Its upstream regulatory region shows no sequence similarity to the corresponding region in Escherichia coli or other Enterobacteriaceae, and yet we show that this operon is regulated by transcription attenuation. The translation of a BCAA-rich peptide encoded upstream of the structural genes provides an adaptive response similar to the E. coli canonical model. This study of a nonmodel Gram-negative organism highlights the mechanistic conservation of transcription attenuation despite the absence of primary sequence conservation. IMPORTANCE This study analyzes the regulation of the biosynthesis of branched-chain amino acids (leucine, valine, and isoleucine) in Vibrio alginolyticus, a marine bacterium that is pathogenic to fish and humans. The results highlight the conservation of the main regulatory mechanism with that of the enterobacterium Escherichia coli, suggesting that such a mechanism appeared early during the evolution of Gram-negative bacteria, allowing adaptation to a wide range of environments.
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