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Bioinformatic analyses of a potential Salmonella-virus-FelixO1 biocontrol phage BPS15S6 and the characterisation and anti-Enterobacteriaceae-pathogen activity of its endolysin LyS15S6

文献类型： 外文期刊

作者： Han, Han 1 ; Li, Xuemin 1 ; Zhang, Tingting 3 ; Wang, Xiaoqing 1 ; Zou, Jiaojiao 1 ; Zhang, Chunxia 1 ; Tang, Huiling; 1 ;

作者机构： 1.Guizhou Educ Univ, Key Lab Guizhou Bioresource Dev & Utilizat, Guiyang 550018, Guizhou, Peoples R China

2.Jiangsu Acad Agr Sci, Int Phage Res Ctr, Nanjing 210014, Jiangsu, Peoples R China

3.Guizhou Normal Univ, Grad Sch, Guiyang 550025, Guizhou, Peoples R China

关键词： Gram-negative bacteria; Genome; Glycoside hydrolase; Epsilon-poly-l-lysine; Outer membrane permeabiliser

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.271； 五年影响因子：2.707 ）

ISSN： 0003-6072

年卷期： 2019 年 112 卷 11 期

页码：

收录情况： SCI

摘要： Foodborne Enterobacteriaceae pathogens, especially Salmonella, still seriously threaten food safety. To establish a foundation for further developing phage- and endolysin-based methods combating these pathogens, in this study, the newly isolated Salmonella-virus-FelixO1 phage BPS15S6 for biocontrol purposes was characterised by genomic bioinformatic analysis, and then its endolysin LyS15S6 was obtained using a prokaryotic expression system, characterised in vitro and evaluated in the antibacterial efficacy. It was shown that BPS15S6 had an 87,609-bp genome with 130 open reading frames and does not appear to carry known lysogeny-associated genes and other damaging genetic determinants and is unlikely to perform generalised transduction. Furthermore, LyS15S6 was determined to possess the high enzymatic activity of 1,001,000 U mg(-1) and the broad spectrum of lysing 56 tested Gram-negative strains. The assays of thermostability and optimum pH revealed that LyS15S6 was stable up to 40 degrees C and more active at pH 7. Notably, we demonstrate that edible epsilon-poly-l-lysine (EPL) can be used as an outer-membrane permeabiliser to improve the antibacterial performance of endolysins. When combined with 1 mu g ml(-1) EPL, 2 mu M LyS15S6 could cause 3-4 log viable cell reductions of the three tested Enterobacteriaceae pathogens in vitro after 2 h of reaction at 25 degrees C and 2.56 and 3.14 log reductions of Salmonella ATCC13076 after 15 min of reaction at 25 degrees C and 2 h of reaction at 8 degrees C respectively. A new strategy, the combined application of endolysins and edible EPL for combating Enterobacteriaceae pathogens in food, is thus presented in this work.

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