Molecular detection and identification of Brassica juncea phyllody and witches' broom-associated phytoplasmas of group 16SrVI-A in Inner Mongolia, China
文献类型： 外文期刊
作者： Zhang, Lei 1 ; Song, Pei-Ling 2 ; Sun, Ping-Ping 1 ; Zhao, Wen-Jun 3 ; Li, Zi-Qin 2 ; Li, Zheng-Nan 1 ;
作者机构： 1.Inner Mongolia Agr Univ, Coll Hort & Plant Protect, Hohhot 010018, Peoples R China
2.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China
3.Chinese Acad Inspect & Quarantine, Beijing 100176, Peoples R China
关键词： Brassica juncea; Phyllody; Witches'-broom; Phytoplasma; 16SrVI-A
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）
ISSN： 0929-1873
年卷期： 2020 年 156 卷 1 期
页码：
收录情况： SCI
摘要： In September 2017, oilseed mustard (Brassica juncea) showing symptoms of phyllody and witches'-broom, indicative of phytoplasma infection, was observed in Ordos, Inner Mongolia, China. Using universal primers P1/P7, the fragment encompassing phytoplasmal 16S rRNA, 16S-23S internal transcribed spacer region and partial 5 ' end of 23S rRNA genes was amplified; the ribosomal protein (rp) genes (rplV-rpsC region) and the partial elongation factor TU (tuf) gene of phytoplasmas were amplified using primer pairs rpF1C/rp(I)R1A and fTufu/rTufu, respectively. The 16S rRNA, rp and tuf phylogenies suggested that both the B. juncea phyllody and witches'-broom associated phytoplasmas are closely related to members of 16SrVI (clover proliferation) group. Based on in silico restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes, the detected phytoplasmas were classified into 16SrVI-A subgroup. The in silico RFLP pattern is consistent with the actual RFLP pattern of the 16S rRNA genes. This was the first record of 16SrVI-A phytoplasma infecting B. juncea in China, probably the first case of phytoplasma on oilseed mustard as well.
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