CLONING AND EXPRESSION OF CINNAMATE 4-HYDROXYLASE GENE FROM DRYOPTERIS FRAGRANS (L.) SCHOTT
文献类型： 外文期刊
作者： Sun, Lili 1 ; Zhang, Liguo 1 ; Zhang, Xiaofei 1 ; Chen, Lingling 1 ; Bu, Zhigang 1 ; Chang, Ying 1 ; Wang, Fude 1 ; Mu, Y 1 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Harbin 150069, Heilongjiang, Peoples R China
2.Guangxi Univ, Coll Math & Informat Sci, Nanning 530004, Guangxi, Peoples R China
3.Northeast Agr Univ, Lab Plant Res, Coll Life Sci, Harbin 150030, Heilongjiang, Peoples R China
4.Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China
关键词： Gene clone; Dryopteris fragrans; Transformation; Cinnamate 4-hydroxylase; Overexpression
期刊名称：BANGLADESH JOURNAL OF BOTANY （ 影响因子：0.308； 五年影响因子：0.348 ）
ISSN： 0253-5416
年卷期： 2019 年 48 卷 3 期
页码：
收录情况： SCI
摘要： The enzyme cinnamate 4-hydroxylase (C4H, EC 1.14.13.11), which belongs to the cytochrome P450 family, is involved in the phenylpropanoid pathway. Using RACE, full-length cDNA consisting of 2063 nucleotides encoding C4H (designated DfC4H) was isolated from Dryopteris fragrans (L.) Schott. A number of 1527 of nucleotides composed the complete open reading frame (ORF) of the cDNA (GenBank Accession Number KF830705.2). A protein that consisted of 508 amino acids with a calculated molecular mass of 58.1908 kDa was predicted to be encoded by this ORF. The overexpression of DfC4H was observed to increase significantly p-coumaric acid and anthocyanin levels in transgenic Arabidopsis thaliana.
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