文献类型： 外文期刊

作者： Huang, Zhaofeng ¹ ; Lu, Zongzhi ² ; Huang, Hongjuan ¹ ; Li, Wenyu ¹ ; Cao, Yi ¹ ; Wei, Shouhui ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China

2.Jilin Acad Agr Sci, Inst Plant Protect, Changchun 130033, Jilin, Peoples R China

关键词： Nicosulfuron resistance; Acetolactate synthase (ALS); Setaria viridis; Metabolism; Cross resistance; Mutation

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2021 年 179 卷

页码：

收录情况： SCI

摘要： Green foxtail [Setaria viridis (L.) P.Beauv.] is a troublesome grass weed that is widely distributed in maize (Zea mays L.) fields across China. Many populations of S. viridis have evolved resistance to the acetolactate synthase (ALS)-inhibiting herbicide nicosulfuron. The objectives of this research were to confirm nicosulfuron resistance in these populations and to investigate the basis of nicosulfuron resistance. Whole-plant dose-response experiments showed 6 out of 13 S. viridis populations were highly resistance (20-30 times) to nicosulfuron. Sequencing of the ALS gene revealed two amino acid mutations, Asp-376-Glu and Pro-197-Ala, in the nicosulfuron-resistant populations. A malathion pretreatment study revealed that the R376 and R197 subpopulations might have cytochrome P450s-mediated herbicide metabolic resistance. The resistant populations were cross-resistant to imazethapyr but sensitive to topramezone and quizalofop-p-ethyl. This is the first report of resistance to ALS inhibitors conferred by target site mutations (Asp-376-Glu or Pro-197-Ser) and possible cytochrome P450sinvolved metabolism in S. viridis.