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Rapid identification and characterization of genetic loci for defective kernel in bread wheat

文献类型： 外文期刊

作者： Fu, Chao 1 ; Du, Jiuyuan 2 ; Tian, Xiuling 1 ; He, Zhonghu 1 ; Fu, Luping 1 ; Wang, Yue 1 ; Xu, Dengan 1 ; Xu, Xiaoting 1 ;

作者机构： 1.Chinese Acad Agr Sci, Natl Wheat Improvement Ctr, Inst Crop Sci, Beijing 100081, Peoples R China

2.Gansu Acad Agr Sci, Wheat Res Inst, Lanzhou 730070, Gansu, Peoples R China

3.Int Maize & Wheat Improvement Ctr, 12 Zhongguancun South St, Beijing 100081, Peoples R China

关键词： Defective kernel; Grain filling; Rapid QTL mapping; SNP array; Triticum aestivem L

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2019 年 19 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundWheat is a momentous crop and feeds billions of people in the world. The improvement of wheat yield is very important to ensure world food security. Normal development of grain is the essential guarantee for wheat yield formation. The genetic study of grain phenotype and identification of key genes for grain filling are of great significance upon dissecting the molecular mechanism of wheat grain morphogenesis and yield potential.ResultsHere we identified a pair of defective kernel (Dek) isogenic lines, BL31 and BL33, with plump and shrunken mature grains, respectively, and constructed a genetic population from the BL31/BL33 cross. Ten chromosomes had higher frequency of polymorphic single nucleotide polymorphism (SNP) markers between BL31 and BL33 using Wheat660K chip. Totally 783 simple sequence repeat (SSR) markers were chosen from the above chromosomes and 15 of these were integrated into two linkage groups using the genetic population. Genetic mapping identified three QTL, QDek.caas-3BS.1, QDek.caas-3BS.2 and QDek.caas-4AL, explaining 14.78-18.17%, 16.61-21.83% and 19.08-28.19% of phenotypic variances, respectively. Additionally, five polymorphic SNPs from Wheat660K were successfully converted into cleaved amplified polymorphic sequence (CAPS) markers and enriched the target regions of the above QTL. Biochemical analyses revealed that BL33 has significantly higher grain sucrose contents at filling stages and lower mature grain starch contents than BL31, indicating that the Dek QTL may be involved in carbohydrate metabolism. As such, the candidate genes for each QTL were predicated according to International Wheat Genome Sequence Consortium (IWGSC) RefSeq v1.0.ConclusionsThree major QTL for Dek were identified and their causal genes were predicted, laying a foundation to conduct fine mapping and dissect the regulatory mechanism underlying Dek trait in wheat.

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