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Conservative transcription in three steps visualized in a double-stranded RNA virus

文献类型： 外文期刊

作者： Cui, Yanxiang 1 ; Zhang, Yinong 1 ; Zhou, Kang 1 ; Sun, Jingchen 2 ; Zhou, Z. Hong 1 ;

作者机构： 1.Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA

2.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA

3.South China Agr Univ, Subtrop Sericulture & Mulberry Resources Protect, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China

期刊名称：NATURE STRUCTURAL & MOLECULAR BIOLOGY （ 影响因子：15.369； 五年影响因子：14.868 ）

ISSN： 1545-9993

年卷期： 2019 年 26 卷 11 期

页码：

收录情况： SCI

摘要： Endogenous RNA transcription characterizes double-stranded RNA (dsRNA) viruses in the Reoviridae, a family that is exemplified by its simple, single-shelled member cytoplasmic polyhedrosis virus (CPV). Because of the lack of in situ structures of the intermediate stages of RNA-dependent RNA polymerase (RdRp) during transcription, it is poorly understood how RdRp detects environmental cues and internal transcriptional states to initiate and coordinate repeated cycles of transcript production inside the capsid. Here, we captured five high-resolution (2.8-3.5 angstrom) RdRp-RNA in situ structures-representing quiescent, initiation, early elongation, elongation and abortive states-under seven experimental conditions of CPV. We observed the 'Y'-form initial RNA fork in the initiation state and the complete transcription bubble in the elongation state. These structures reveal that de novo RNA transcription involves three major conformational changes during state transitions. Our results support an ouroboros model for endogenous conservative transcription in dsRNA viruses.

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