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Molecular characterization and functional analysis of Akirin from black tiger shrimp (Penaeus monodon)

文献类型： 外文期刊

作者： Peng, Chao 1 ; Xie, Dongchang 1 ; Zhao, Chao 1 ; Xu, Haidong 1 ; Fan, Sigang 1 ; Yan, Lulu 1 ; Wang, Pengfei 1 ; Qiu, Lih 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr, Guangzhou, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Coll Aqualife Sci & Technol, Shanghai, Peoples R China

3.Minist Agr, Key Lab Aquat Genom, Beijing, Peoples R China

关键词： Akirin; Antimicrobial peptides; Penaeus monodon; Vibrio parahaemolyticus; RNAi

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2019 年 94 卷

页码：

收录情况： SCI

摘要： Akirin, which are members of the NF-kappa B signaling pathway, play critical roles in regulating the expression of antimicrobial peptides. In the present study, the Akirin gene from Penaeus monodon was identified from a transcriptome database and designated as PmAkirin. The complete sequence of the PrnAkirin cDNA was 1508 bp, encoding a protein of 213 amino acids, and it showed 99% amino acid identity to the Litopenaeus vannamei Akirin. Two predicted nuclear localization signals (NLSs) were found, and the amino acid sequence alignments showed that PmAkirin was highly conserved at the N-terminus and C-terminus. PmAkirin expression was found to be the highest in the hemolymph, followed by the heart, gill, stomach, hepatopancreas, intestine, and muscle. When challenged with Vibrio parahaemolyticus infection, the PmAkirin mRNA and three antimicrobial peptides (AMPs: PmALF2, PrnALF3, and PmCrus4) were upregulated. However, another five AMPs (PmALF6, PmCrus1, PmPEN3a, PrnPEN3b, and PmPEN5) were downregulated by V. parahaemolyticus infection. Silencing PmAkirin by dsRNA significantly decreased the expression of the eight AMPs, which lead to an increase in the blood concentration of V. parahaemolyticus and higher mortality in the shrimp. In contrast, the overexpression of PmAkirin significantly increased the expression of the eight AMPs, which led to a reduction in the blood concentration of V. parahaemolyticus and promoted the survival of the shrimp. Taken together, we concluded that PmAkirin plays an important role in regulating the expression of AMPs in black tiger shrimp to defend against V. parahaemolyticus infection.

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