Establishment of a cell line from egg of rare minnow Gobiocypris rarus for propagation of grass carp reovirus genotype II
文献类型： 外文期刊
作者： Liu, Shixu 1 ; Wang, Yingying 1 ; Chen, Jiaming 3 ; Wang, Qing 1 ; Chang, Ouqin 1 ; Zeng, Weiwei 1 ; Bergmann, Sven M. 4 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab Fishery Drug Dev, Peal River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol,Minist Agr, Guangzhou 510380, Guangdong, Peoples R China
2.Tianjin Agr Univ, Coll Fisheries, Tianjin 300384, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
4.Fed Res Inst Anim Hlth, FLI, Inst Infectol, D-17493 Greifswald, Inset Riems, Germany
关键词： Grass carp reovirus (GCRV); Gobiocypris rarus; Cell line; GrE; Genotype II
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）
ISSN： 0882-4010
年卷期： 2019 年 136 卷
页码：
收录情况： SCI
摘要： The rare minnow, Gobiocypris rarus, is small experimental fish proven to be sensitive to Grass Carp Reovirus (GCRV) infection. In present study we established a new cell (GrE) from eggs of G. rarus. GrE cells grew well at 28 degrees C in M199 medium containing 10% fetal bovine serum, and has been subcultured for over 70 passages. Chromosome analysis indicated that 40% of the cells were diploid 2n = 66 while the chromosome number of the fish is 2n = 50. Viral replication in GrE cells was confirmed by transmission electron microscopy, immunofluorescence assays and virus titration experiments. GrE cells and Cyenopharyngodon idellus kidney cells were infected with two GCRV genotypes while the virus copies of GCRV II in GrE peaked at 2.25 x 10(5) on 12th dpi. In vivo challenge experiments using GCRV I and II isolates at generations 1 and 20 indicated that GCRV II reproduce similar symptoms and histopathological changes of the disease in the rare minnow. These results indicated that GrE is permissive for GCRV genotype II propagation and can be used for pathogenesis studies and vaccine development of the predominant genotype of GCRV.
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