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Sucrose alleviated programmed cell death in broccoli after harvest

文献类型： 外文期刊

作者： Li, Yanxia 1 ; Cao, Shifeng 2 ; Han, Airu 1 ; Wang, Hongfei 1 ; Wei, Yingying 1 ; Shao, Xingfeng 1 ; Gao, Haiyan 3 ; Xu, 1 ;

作者机构： 1.Ningbo Univ, Coll Food & Pharmaceut Sci, Key Lab Anim Prot Food Proc Technol Zhejiang Prov, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Zhejiang, Peoples R China

2.Zhejiang Wanli Univ, Coll Biol & Environm Sci, Key Lab Fruits & Vegetables Postharvest & Proc Te, Ningbo 315100, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Food Sci Inst, Key Lab Fruits & Vegetables Postharvest & Proc Te, 298 Middle Desheng Rd, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Programmed cell death; sucrose; DNA fragmentation; caspase-like activities

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：5.537； 五年影响因子：5.821 ）

ISSN： 0925-5214

年卷期： 2020 年 160 卷

页码：

收录情况： SCI

摘要： The effect of sucrose treatment on morphological and biochemical features of programmed cell death (PCD) in broccoli florets was explored. The apoptosis of untreated and treated cells was evaluated by flow cytometry (FCM) and by laser scanning confocal microscopy (LSCM). DNA ladders, resulting from the cleavage of nuclear DNA into oligonucleosomal fragments in PCD, were evaluated by gel electrophoresis. DNA frag-mentation and nuclear DNA condensation were further confirmed by using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labeling (TUNEL). The results revealed that sucrose treatment inhibited DNA degradation, and delayed the early apoptosis of broccoli cell as demonstrated with FCM and LSCM. DNA laddering was observed in control broccoli florets, which could not be detected in sucrose-treated samples. In addition, the caspase-like activities in broccoli cells were suppressed under sucrose treatment. Taken together, our results suggested that PCD was delayed in sucrose-treated broccoli florets, which provided a new model to investigate the mechanism of sugar in regulating PCD during postharvest storage.

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