Screening and activity identification of an anti-idiotype nanobody for Bt Cry1F toxin from the camelid naive antibody phage display library
文献类型： 外文期刊
作者： Hao, Jia 1 ; Li, Yihang 1 ; Wang, Jingxuan 1 ; Xu, Chongxin 2 ; Gao, Meijing 2 ; Chen, Wei 2 ; Zhang, Xiao 2 ; Hu, Xiaoda 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Key Lab Food Qual & Safety Jiangsu Prov, Key Lab Control Technol & Stand Agriprod Safety &, State Key Lab Breeding Base,Minist Agr, Nanjing, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Agr Prod Qual Safety & Nutr Res, Nanjing, Jiangsu, Peoples R China
关键词： Bacillus thuringiensis; Cry1F toxin; anti-idiotype nanobody; Ostrinia furnacalis (Guenee); brush border membrane vesicles (BBMV)
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
ISSN： 0954-0105
年卷期： 2020 年 31 卷 1 期
页码：
收录情况： SCI
摘要： Anti-idiotypic antibody technology has been widely developed. Here, we prepared the F(ab')(2) fragments of the Cry1F toxin polyclonal antibody as Ab1and screened an anti-idiotype nanobody (VHH) for Bt Cry1F toxin from the camelid naive antibody phage display library. After three cycles of panning, six clones were isolated and subjected to indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) for the detection of the Cry1F toxin. A competition inhibition rate of 47.00% exists between the anti-idiotype nanobody with the highest activity (5B) and Cry1F toxin for F(ab')(2) fragments. The binding abilities between the anti-idiotypic nanobody 5B and brush border membrane vesicles (BBMV) of Ostrinia furnacalis (Guenee) are also good. The anti-idiotypic nanobody 5B expressed through the prokaryotic system has the same activity as in phage form, and mortality compared to controls could reach 23.3% (P < 0.05). The material has the potential to simulate the Cry1F toxin to a certain extent.
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