您好，欢迎访问福建省农业科学院 机构知识库！
福建省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Ethylene and Benzaldehyde Emitted from Postharvest Tomatoes Inhibit Botrytis cinerea via Binding to G-Protein Coupled Receptors and Transmitting with cAMP-Signal Pathway of the Fungus

文献类型： 外文期刊

作者： Lin, Yongwen 1 ; Ruan, Hongchun 3 ; Akutse, Komivi Senyo 4 ; Lai, Baochun 1 ; Lin, Yizhang 1 ; Hou, Youming 1 ; Zhong, 1 ;

作者机构： 1.Fujian Agr & Forestry Univ Fujian, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350013, Fujian, Peoples R China

2.Fujian Agr & Forestry Univ Fujian, Coll Hort, Fuzhou 350013, Fujian, Peoples R China

3.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350013, Fujian, Peoples R China

4.Int Ctr Insect Physiol & Ecol, Nairobi 3077200100, Kenya

关键词： postharvest disease; volatile emissions; signaling pathway; gray mold; Lycopersicon esculentum (tomato); G protein-coupled receptors

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 49 期

页码：

收录情况： SCI

摘要： Tomato storage conditions are difficult largely due to Botrytis cinerea infection which causes gray mold disease. However, the effects of the volatile organic compounds (VOCs) emitted by postharvest tomatoes on this fungus remain unclear. We analyzed the effects of tomato-emitted VOCs on B. cinerea pathogenicity, germination, and hyphal growth with bioassay, predicted the causative active compounds by principle component analysis, identified G-protein-coupled receptors (GPCRs) which captured chemical signals in the B. cinerea genome by stimulating molecular docking, tested the binding affinities of these receptors for the active compounds by fluorescence binding competition assay, and identified an associated signaling pathway by RNA interfere. The VOCs emitted by postharvest tomatoes inhibited B. cinerea; ethylene and benzaldehyde were the active compounds causing this effect. One of the identified GPCRs in B. cinerea, BcGPR3, bound tightly to both active compounds. Two genes associated with the cAMP signaling pathway (BcRcn1 and BcCnA) were downregulated in wild-type B. cinerea exposed to the active compounds, as well as in the Delta Bcgpr3 B. cinerea mutant. Exposure to postharvest tomato VOCs reduces B. cinerea pathogenicity due to ethylene and benzaldehyde volatiles. The BcGPR3 protein is inactivated by the active compounds, and thus fails to transmit signals to the cAMP pathway, thereby inhibiting B. cinerea.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：福建省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台