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MicroRNA-22 inhibits proliferation and promotes differentiation of satellite cells in porcine skeletal muscle

文献类型： 外文期刊

作者： Hong Quyen Dang 1 ; Xu Gu-li 1 ; Hou Lian-jie 1 ; Xu Jian 1 ; Hong Guang-liang 1 ; Hu, Chingyuan 3 ; Wang Chong 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Guangdong, Peoples R China

2.Bac Giang Agr & Forestry Univ, Bac Giang 26000, Vietnam

3.Univ Hawaii Manoa, Coll Trop Agr & Human Resources, Dept Human Nutr Food & Anim Sci, Honolulu, HI 96822 USA

关键词： miR-22; skeletal muscle; porcine satellite cells; proliferation; differentiation

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2020 年 19 卷 1 期

页码：

收录情况： SCI

摘要： Pig is an important economic animal in China. Improving meat quality and meat productivity is a long time issue in animal genetic breeding. MicroRNAs (miRNAs) are short non-coding RNAs that participate in various biological processes, such as muscle development and embryogenesis. miR-22 differentially expresses in embryonic and adult skeletal muscle. However, the underlying mechanism is unclear. In this study, we investigated miR-22 function in proliferation and differentiation of porcine satellite cells (PSCs) in skeletal muscle. Our data show that miR-22 expressed in both proliferation and differentiated PSCs and is significantly upregulated (P<0.05) during differentiation. After treated with the miR-22 inhibitor, PSCs proliferation was significantly increased (P<0.05), as indicated by the up-regulation (P<0.01) of cyclin D1 (CCND1), cyclin B1 (CCNB1) and down-regulation (P<0.05) of P21. Conversely, over-expression of miR-22 resulted in opposite results. Differentiation of PSCs was significantly suppressed (P<0.05), evidenced by two major myogenic markers: myogenin (MyoG) and myosin heavy chain (MyHC), after transfecting the PSCs with miR-22 inhibitor. Opposite results were demonstrated in the other way around by transfection with miR-22 mimics. In conclusion, the data from this study indicated that miR-22 inhibited the PSCs proliferation but promoted their differentiation.

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