文献类型： 外文期刊

作者： He, J. F. ¹ ; Fang, Y. Y. ¹ ; Lu, Z. X. ³ ; Wang, L. W. ¹ ; Zhao, X. Q. ¹ ; Fu, X. F. ¹ ; Zhao, J. ² ; Liu, H. K. ¹ ;

作者机构： 1.Inner Mongolia Acad Agr & Anim Husb Sci, Biotechnol Res Ctr, Hohhot 010030, Peoples R China

2.Inner Mongolia Med Univ, Affiliated Hosp, Reprod Ctr, Hohhot 010050, Peoples R China

3.Agr & Agri Food Canada, Lethbridge Res Ctr, Lethbridge, AB T1J 4B1, Canada

关键词： carbon metabolism; drought and salinity tolerance; heat shock proteins; hormone-related genes; NaCl; RNA-Seq; transcription factors

期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）

ISSN： 0006-3134

年卷期： 2019 年 63 卷

页码：

收录情况： SCI

摘要： Haloxylon ammodendron (C.A. Mey.), an endangered desert tree with excellent drought and salinity tolerance, provides a unique genotype to characterize and understand the tolerance mechanisms. In this study, four RNA-Seq libraries were constructed and sequenced from H. ammodendron under salinity stress. Total 12 027 differentially expressed genes (DEGs) were identified, in which 4 023, 3 517, 4 487 genes were differentially expressed under light salinity stress (200 mM NaCl), moderate salinity stress (400 mM NaCl), and severe salinity stress (800 mM NaCl), respectively. The up-regulated DEGs included several transcription factors (e.g., MYB and bHLH), hormone-related genes (e.g., cytochrome P450), protein kinases (e.g., Atpk2-Atpk19 like), and genes involved in carbon metabolism (e.g., UDP glycosyltransferase), osmotic regulation (e.g., proline transporter), and ubiquitin proteasome system (e.g., ubiquitin-conjugating enzymes). Heat shock proteins were identified as positive regulators of salinity tolerance in H. ammodendron. The expression patterns of 13 DEGs verified by real-time quantitative PCR were identically consistent with the variations in transcript abundance identified by RNA-Seq. Our results provide new insights into molecular mechanism of H. ammodendron in response to salinity stress.