文献类型： 外文期刊

作者： Jiang, Y. ¹ ; Ogunade, I. M. ¹ ; Arriola, K. G. ¹ ; Pech-Cervantes, A. A. ¹ ; Kim, D. H. ¹ ; Li, X. ¹ ; Xue, Y. L. ³ ; Vy ¹ ;

作者机构： 1.Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA

2.Kentucky State Univ, Div Food & Anim Sci, Frankfort, KY 40601 USA

3.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China

4.RDA, Natl Inst Anim Sci, Cheonan 31000, South Korea

5.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai, Peoples R China

关键词： aflatoxin; sequestering agent; bentonite clay; rumen fermentation

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2020 年 103 卷 2 期

页码：

收录情况： SCI

摘要： Aflatoxin is a potent carcinogen commonly found in animal feeds that can impair rumen fermentation at high concentrations; however, its effects at physiologically relevant concentrations are unknown. This study examined the effects of aflatoxin B-1 (AFB(1)), with or without bentonite clay (CL) and Saccharomyces ceretrisiae fermentation product (SCFP) based sequestering agents on in vitro rumen fermentation and digestibility of a dairy cow TMR. Corn silage-based TMR (0.5 g, 17.3% crude protein and 1.67 Mcal/kg of net energy for lactation) was incubated in a rumen fluid-buffer inoculum (1:2 ratio; 50 mL) with the following treatments: (1) no additives (control); (2) control + 0.75 mu g/L AFB(1) (T); (3) T + 80 mg/L sodium bentonite clay (CL; Astra-Ben-20, Prince Agri Products Inc., Quincy, IL); or (4) CL + 14 mg/L SCFP (CL+SCFP; Diamond V, Cedar Rapids, IA). Ruminal fluid was collected 3 h after the morning feeding from 3 cannulated cows fed the same TMR, and rumen fluid from individual cows was used to prepare separate inocula. Each treatment was incubated in duplicate at 39 degrees C for 0, 4, 8, 16, and 24 h in each of 3 runs. Adding T reduced total volatile fatty acid (VFA) concentration after 4 and 8 h and molar proportion of propionate after 4 and 24 h of incubation relative to control. Adding sequestering agents (CL and CL I SCFP) with T did not affect total VFA concentration after 4 or 8 h, but increased total VFA after 16 h and tended to increase molar proportion of propionate after 24 It compared with T. At 24 h, T had lower DM digestibility and higher NH3-N concentration compared with the control. Thus, AFB(1), even at very low concentration (0.75 mu g/L), had detrimental effects on rumen fermentation and subsequently DM digestibility of the TMR. Adding sequestering agents did not prevent negative effects of T on rumen fermentation within 8 h of incubation; however, sequestering agents were effective after 16 h of incubation.