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Improvement of Salt Tolerance on Escherichia coli by Expression of Agglutinin from Amygdalus mira

文献类型： 外文期刊

作者： Xu, Liping 1 ; Ji, Ximei 1 ; Jin, Guangze 2 ; Guan, Fachun 3 ; Luo, Qiuxiang 1 ; Meng, Fanjuan 1 ;

作者机构： 1.Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China

2.Northeast Forestry Univ, Sch Forestry, Key Lab Sustainable Forest Ecosyst Management, Minist Educ, Harbin 150040, Peoples R China

3.Jilin Acad Agr Sci, Changchun 130033, Peoples R China

4.Tibet Agr & Anim Husb Coll, Nyingchi 860000, Peoples R China

5.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Minist Educ, Key Lab Saline Alkaline Vegetat Ecol Restorat Sav, Harbin 150040, Peoples R China

关键词： Amygdalus mira (Koehne) Yu et Lu; Agglutinin; Expression; Purification; Salt stress; Tolerance

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2020 年 23 卷 2 期

页码：

收录情况： SCI

摘要： Amygdalus mira (Koehne) Yu et Lu, also called Tibetan peach, is a wild species belonging to the genus Amygdalus, from family Roseceae. It is distributed in Tibet and possesses tolerance to extreme environment, such as salt, cold and drought and also possesses resistance to disease. In present research, we cloned the AmAGG gene in the root of A. mira and analyzed its expression pattern in drought roots of A. mira (Koehne) Yu et Lu by qRT-PCR. The result showed the expression of AmAGG increased under drought and decreased after re-watered, indicating that the AmAGG was induced by drought. Accordinlgy, the recombined AmAGG protein (pET-AmAGG) was induced and purified from E. coli strain (Rossetta). The optimal expression temperature was 28 degrees C, the optimal induction time was 6 h and the optimum concentration of IPTG was 0.1 mM, through which the protein was induced and expressed with soluble form in Rossetta strain. The salt tolerance of E. coli expressing the agglutinin was estimated by measuring at OD600 (optical density at 600 nm) value of Rossetta by supplementing different concentrations of NaCl into the culture media. The results indicated that Rossetta strain harboring the AmAGG protein was more tolerant to salt stress than that of harbored pET -15b empty vector, revealed the AmAGG might improve the salt tolerance of Rossetta cells. The data suggested that AmAGG plays a crucial role in tolerance against drought and salinity stresses. (C) 2020 Friends Science Publishers

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