RNA interference-mediated knockdown of a cytochrome P450 gene enhanced the toxicity of alpha-cypermethrin in xanthotoxin-fed larvae of Spodoptera exigua (Hubner)
文献类型： 外文期刊
作者： Hafeez, Muhammad 1 ; Liu, Sisi 3 ; Yousaf, Hafiz Kamran 4 ; Jan, Saad 5 ; Wang, Rui-Long 6 ; Fernandez-Grandon, G. 1 ;
作者机构： 1.Huazhong Agr Univ Wuhan, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Hubei, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Qual & Safety Agroprod, Hangzhou 310021, Peoples R China
3.Huazhong Agr Univ Wuhan, Coll Sci, Wuhan 430070, Hubei, Peoples R China
4.China Agr Univ, Coll Plant Protect Dept Entomol, Beijing 100193, Peoples R China
5.Bacha Khan Univ Charsadda, Dept Agr Entomol Sect, Charsadda, Pakistan
6.South China Agr Univ, Key Lab Agroenvironm Trop, Minist Agr, Guangzhou 510642, Peoples R China
7.Univ Greenwich, Nat Resources Inst, Chatham Maritime, Chatham ME4 4TB, Kent, England
8.Pir Mehr Ali Shah Arid Agr Univ Rawalpindi, Dept Entomol, Rawalpindi, Pakistan
9.Huazhong Agr Univ, Coll Plant Sci & Technol, MOA Key Lab Crop Ecophysiol & Farming Syst Middle, Wuhan 430070, Hubei, Peoples R China
10.Bacha Khan Univ Charsadda, Dept Agr 24420, Entomol Sect, Charsadda, Pakistan
11.Bahauddin Zakariya Univ Multan, Fac Agr Sci & Technol, Dept Plant Breeding & Genet, Punjab 60000, Pakistan
关键词： Beet armyworm; Plant secondary metabolites; Insecticide sensitivity; Cytochrome P450 monooxygenase; RNA interference
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）
ISSN： 0048-3575
年卷期： 2020 年 162 卷
页码：
收录情况： SCI
摘要： The beet armyworm (Spodoptera exigua) is a highly polyphagous agricultural pest that is distributed worldwide. However, the adaptive mechanisms of S. exigua for various insecticides and defensive substances in host plants are unknown. Insect P450 monooxygenases play an important role in the detoxification of plant toxins and insecticides, leading to insecticides resistance. We investigated the induced effects of xanthotoxin exposure on detoxification enzyme activity and larval tolerance to alpha-cypermethrin in S. exigua. Our results showed that the lethal concentration (LC50) of alpha-cypermethrin for xanthotoxin-exposed larvae was 2.1-fold higher than in the control. Moreover, cytochrome P450 enzyme activity was significantly elevated by upregulation of P450 genes in treated larvae. RT-qPCR results showed that CYP9A10 expression level was significantly increased in all treatments, while maximal expression level was observed in xanthotoxin + alpha-cypermethrin-fed larvae. RNAi-mediated silencing of CYP9A10 further increased mortality by 18%, 26% and 35% at 48 h and by 27%, 43% and 55% at 72 h when larvae were exposed to diets containing chemicals as compared to the control. The results show that CYP9A10 might play an important role in xanthotoxin and alpha-cypermethrin detoxification in S. exigua. RNAi-mediated silencing could provide an effective synergistic agent for pest control or insecticide resistance management.
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