Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis
文献类型： 外文期刊
作者： Shang, Xiaomei 1 ; Ma, Aijun 1 ; Wang, Xin'an 1 ; Xia, Dandan 1 ; Zhuang, Jiao 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Shandong Key Lab Marine Fisheries Biotechnol & Ge, Qingdao 266071, Peoples R China
3.Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
5.Tianjin Univ Sci & Technol, Coll Marine & Environm Sci, Tianjin 300457, Peoples R China
关键词： transient receptor potential vanilloid 4 (TRPV4); Cynoglossus semilaevis; gene expression; free neuromasts; in situ hybridization
期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）
ISSN： 2096-5508
年卷期： 2020 年 38 卷 1 期
页码：
收录情况： SCI
摘要： The transient receptor potential vanilloid 4 (TRPV4), another Ca2+ entry channel, belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in mammals. However, our understanding of the TRPV4 in fish remains poor. Therefore, we studied the TRPV4 gene from Cynoglossus semilaevis, named CsTRPV4 that encodes a putative protein of 870 amino acids common in structure and characteristic of mammalian TRPV4, including the domains of ANK repeats, six TM, TRP domain, and CaMBD. The CsTRPV4 was expressed ubiquitously in examined tissues: higher expression in the heart, spleen, testis, and eye, but lower expression in kidney and liver. Surprisingly, the expression of CsTRPV4 in lateral line was significantly higher than in many other tissues as the CsTRPV4 was expressed significantly in the free neuromasts. In addition, CsTRPV4 in the free neuromast from the larval fish was significantly expressed in the hair cells of the free neuromasts. Therefore, the free neuromasts can act as a mechano-sensor to the mechanical stimulation in molecular level in C. semilaevis, which lays a foundation for further study of the functions of the free neuromasts.
- 相关文献
作者其他论文 更多>>
-
Development and evaluation of a haplotype reference panel for low-coverage whole genome sequencing genotype imputation in turbot (Scophthalmus maximus)
作者：Cao, Junwen;Huang, Zhihui;Ma, Aijun;Jiang, Yuhang;Zhang, Hao;Wang, Xinan;Liu, Zhifeng;Cao, Junwen;Huang, Zhihui;Ma, Aijun;Jiang, Yuhang;Zhang, Hao;Wang, Xinan;Liu, Zhifeng;Cao, Junwen;Huang, Zhihui;Ma, Aijun;Jiang, Yuhang;Zhang, Hao;Wang, Xinan;Liu, Zhifeng;Cao, Junwen;Huang, Zhihui;Ma, Aijun;Jiang, Yuhang;Zhang, Hao;Wang, Xinan;Liu, Zhifeng;Cao, Junwen;Jiang, Yuhang;Cao, Junwen;Huang, Zhihui;Ma, Aijun;Jiang, Yuhang;Zhang, Hao;Wang, Xinan;Liu, Zhifeng;Zhang, Rongchao;Xu, Rongjing
关键词：Scophthalmus maximus; Turbot; Haplotype reference panel; Genotype imputation
-
Thermal tolerance of turbot based on cardiac performance and analysis of molecular regulatory mechanisms
作者：Jiang, Yuhang;Huang, Zhihui;Ma, Aijun;Cao, Junwen;Zhang, Hao;Zhao, Yinuo;Hu, Yan;Jiang, Yuhang;Cao, Junwen;Zhao, Yinuo;Jiang, Yuhang;Huang, Zhihui;Ma, Aijun;Cao, Junwen;Zhang, Hao;Zhao, Yinuo;Hu, Yan;Liu, Ming
关键词：
Scophthalmus maximus (L.); Thermal stress; beta-AR; Heart rate; Cardiac performance -
Analysis of pigment cell differences and carotenoid content in the skin of golden turbot (Scophthalmus maximus) with color variation
作者：Liu, Zhifeng;Yang, Mingchao;Shi, Yuelei;Yan, Pengfei;Sun, Zhibin;Zhong, Junlian;Huang, Zhihui;Wang, Hui;Ma, Aijun;Liu, Zhifeng;Sun, Zhibin;Huang, Zhihui;Ma, Aijun;Yang, Mingchao;Wang, Hui
关键词：Scophthalmus maximus; Golden body color; Pigment cell; Carotenoid
-
Genetic Parameters of the Growth Rate, Survival Rate and Feed Efficiency Ratio of Turbot (Scophthalmus maximus) at an Early Growth Stage
作者：Gou, Donghui;Wang, Yilin;Wang, Xinan;Sun, Zhibin;Ma, Aijun;Gou, Donghui;Wang, Yilin;Wang, Xinan;Sun, Zhibin;Ma, Aijun;Wang, Yilin;Wang, Xinan;Sun, Zhibin;Ma, Aijun
关键词：turbot; growth rate; survival rate; feed efficiency ratio; heritability; genetic correlation
-
pparβ regulates lipid catabolism by mediating acox and cpt-1 genes in Scophthalmus maximus under heat stress
作者：Zhao, Tingting;Ma, Aijun;Huang, Zhihui;Liu, Zhifeng;Sun, Zhibin;Zhu, Liguang;Chang, Haowen;Ma, Aijun;Huang, Zhihui;Liu, Zhifeng;Sun, Zhibin;Zhu, Liguang;Chang, Haowen
关键词：Scophthalmus maximus; ppar beta; High temperature; Lipid catabolism; RNAi
-
Genome-wide association study of feed conversion ratio in turbot (Scophthalmus maximus) based on genome resequencing
作者：Liu, Zhifeng;Chang, Haowen;Xu, Fei;Zhao, Haichi;Zhu, Liguang;Sun, Zhibin;Yang, Mingchao;Wang, Xinan;Ma, Aijun;Liu, Zhifeng;Chang, Haowen;Xu, Fei;Zhao, Haichi;Zhu, Liguang;Sun, Zhibin;Yang, Mingchao;Wang, Xinan;Ma, Aijun;Liu, Zhifeng;Chang, Haowen;Xu, Fei;Zhao, Haichi;Zhu, Liguang;Sun, Zhibin;Yang, Mingchao;Wang, Xinan;Ma, Aijun;Liu, Zhifeng;Chang, Haowen;Xu, Fei;Zhao, Haichi;Zhu, Liguang;Sun, Zhibin;Yang, Mingchao;Wang, Xinan;Ma, Aijun;Liu, Zhifeng;Zhao, Haichi;Sun, Zhibin;Wang, Xinan;Ma, Aijun;Xu, Fei
关键词：Turbot; Feed conversion ratio (FCR); Genome-wide association study (GWAS); Genome resequencing
-
Genotype by Temperature Interaction for Plasma Physiological Indexes in Turbot (Scophthalmus maximus) under Acute Heat Stress-Exploring a Method for Screening Physiological Biomarkers of Nontoxic Stress in Aquatic Environments
作者：Wang, Xinan;Meng, Zhen;Ma, Aijun;Wang, Xinan;Meng, Zhen;Ma, Aijun
关键词：Genotype; temperature; temperature ? genotype interactions; plasma physiological parameters; aquatic environments; Scophthalmus maximus
