Establishment of a brain cell line obtained from hybrids of Channa argus x Channa maculata for the detection of tilapia lake virus
文献类型： 外文期刊
作者： Wang, Yingying 1 ; Li, Zhili 3 ; Wang, Qing 1 ; Zeng, Weiwei 1 ; Li, Yingying 1 ; Yin, Jiyuan 1 ; Bergmann, Sven M. 4 ; Z 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Key Lab Fishery Drug Dev, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510225, Guangdong, Peoples R China
4.Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Infectol, Sudufer 10, D-17493 Greifswald, Germany
关键词： Cell line; Brain; Hybrid snakehead; Tilapia lake virus
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）
ISSN： 0882-4010
年卷期： 2020 年 138 卷
页码：
收录情况： SCI
摘要： A brain cell line (CAMB) derived from hybrid snakehead (Channa argus (male) x Channa maculata (female)) was established by trypsin and collagenase combined digestion. The culturing conditions and cell biological characteristics were systematically studied. For growth of the cells, M199 medium containing 10% fetal bovine serum was used and at 27 degrees C incubated. Based on morphological analysis, CAMB cells were confirmed to be epithelial. The cell line has been subcultured more than 80 times since its initial primary culture. Chromosome analysis revealed that CAMB cells had an abnormal chromosome number 2n = 64, whereas the chromosome number in the hybrid snakehead was 45. The suitability of CAMB for tilapia lake virus (TiLV) was demonstrated. A CPE was observed after infection with TiLV-2017A. The highest TiLV titer was observed after 12 days post infection (dpi) and reached 107.2 TCID50/mL. The virus replication was confirmed by electron microscopic observations. Additionally, immunofluorescence assay confirmed the presence of TiLV-2017A after infection of CAMB. Therefore, CAMB cells can be a useful tool for the investigation of the pathogenesis of the TiLV induced disease in tilapia.
- 相关文献
作者其他论文 更多>>
-
Identification of a novel strain of carp edema virus affecting Songpu mirror carp in Liaoning, China
作者：Shi, Wen;Han, Bing;He, Huilin;Feng, Ruixue;Guo, Siyi;Ren, Xuanyu;Huang, Jinshan;Lin, Xu;Liu, Min;Yin, Jiyuan;Lv, Xiaonan
关键词：Carp edema virus disease; Carp edema virus; Detection; Songpu mirror carp; Genotype IIa
-
Cyprinid herpesvirus 3 replication is inhibited via decreased ORF3 promoter activity mediated by multiple cellular transcription factors
作者：Liu, Donghai;Wang, Yingying;Li, Yingying;Mo, Xubing;Yin, Jiyuan;Wang, Qing;Zheng, Shucheng;Liu, Donghai
关键词：Cyprinid herpesvirus 3; Koi herpesvirus; ORF3; Promoter; Transcription factors
-
Identification of a novel birnavirus strain in diseased Siniperca chuatsi and transcriptome profiling of virus-infected MFSB cells
作者：Cui, Hongye;Chen, Haiyue;Wang, Yuhui;Wang, Yaoda;Hu, Tianmei;Wu, Baozhou;Hao, Shuguang;Zhang, Jiping;Liu, Yu;Zeng, Weiwei;Xu, Liwen;Hu, Xiong
关键词：Siniperca chuatsi; Birnavirus; Genome sequence; SCBV; Immune response
-
Enhanced virulence of Acinetobacter johnsonii at low temperatures induces acute immune response and systemic infection in American bullfrogs (Aquarana catesbeiana)
作者：Lin, Han;Sun, Jingyang;Li, Hua;Chen, Kesong;Qin, Zhendong;Jiang, Biao;Li, Wei;Su, Youlu;Lin, Li;Liu, Chun;Wang, Qing;Wang, Qing
关键词：Cold stress; Virulence genes; Amphibian disease; Immune response; Systemic infection
-
Genetic Diversity and Population Structure of the Chinese Three-Keeled Pond Turtle (Mauremys reevesii)
作者：Zhou, Chenyao;Zhu, Xinping;Zhao, Bo;Zhou, Chenyao;Xu, Haoyang;Liu, Haiyang;Li, Jipeng;Li, Wei;Hong, Xiaoyou;Chen, Chen;Ji, Liqin;Zhu, Xinping;Liu, Xiaoli;Xu, Haoyang;Li, Jipeng;Li, Wei;Zhu, Xinping;Liu, Xiaoli
关键词：
Mauremys reevesii ; population genomics; population structure; whole-genome resequencing -
A chromosome-level reference genome assembly of the Small snakehead (Channa asiatica)
作者：Liu, Haiyang;Liu, Jiafeng;Cui, Tongxin;Zhang, Jin;Luo, Qing;Fei, Shuzhan;Chen, Kunci;Zhu, Xinping;Ou, Mi;Zhao, Jian;Liu, Haiyang;Liu, Jiafeng;Zhang, Jin;Cui, Tongxin
关键词：
-
Frog virus 3: Prevalence survey and quantitative analysis in American bullfrogs using TaqMan MGB probe real-time fluorescence quantitative PCR
作者：Li, Hua;Liu, Xiaobing;Sun, Jingyang;Lin, Han;Chen, Kesong;Hu, Yueming;Jiang, Mengzhen;Jiang, Biao;Li, Wei;Su, Youlu;Liu, Chun;Wang, Qing;Wang, Qing
关键词：Aquarana catesbeiana; Frog virus 3; TaqMan-MGB real-time PCR; Virus load; Prevalence survey
