Comprehensive transcriptome analysis of reference genes for fruit development of Euscaphis konishii
文献类型： 外文期刊
作者： Yang, Cheng-Long 1 ; Yuan, Xue-Yan 2 ; Zhang, Jie 1 ; Sun, Wei-Hong 2 ; Liu, Zhong-Jian 2 ; Zou, Shuang-Quan 2 ;
作者机构： 1.Fujian Acad Agr Sci, Biotechnol Inst, Fuzhou, Fujian, Peoples R China
2.Fujian Agr & Forestry Univ, Coll Forestry, Fujian Coll & Univ Engn Res Inst Conservat & Util, Fuzhou, Fujian, Peoples R China
3.Fujian Agr & Forestry Univ, Coll Landscape Architecture, Key Lab Natl Forestry & Grassland Adm Orchid Cons, Fuzhou, Fujian, Peoples R China
关键词： Euscaphis konishii; Fruit development; Reference genes; qRT-PCR
期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）
ISSN： 2167-8359
年卷期： 2020 年 8 卷
页码：
收录情况： SCI
摘要： Background. Quantitativereal-time reverse transcriptase polymerase chain reaction is the common method to quantify relative gene expression. Normalizating using reliable genes is critical in correctly interpreting expression data from qRT-PCR. Euscaphis konishii is a medicinal plant with a long history in China, which has various chemical compounds in fruit. However, there is no report describing the selection of reference genes in fruit development of Euscaphis konishii. Methods. We selected eight candidate reference genes based on RNA-seq database analysis, and ranked expression stability using statistical algorithms GeNorm, NormFinder, BestKeeper and ReFinder. Finally, The nine genes related to the anthocyanin synthesis pathway of Euscaphis konishii were used to verify the suitability of reference gene. Results. The results showed that the stability of EkUBC23, EkCYP38 and EkGAPDH2 was better, and the low expression reference genes (EkUBC23 and EkCYP38) were favourable for quantifying low expression target genes, while the high expression reference gene (EkGAPDH2) was beneficial for quantifying high expression genes. In this study, we present the suitable reference genes for fruit development of Euscaphis konishii based on transcriptome data, our study will contribute to further studies in molecular biology and gene function on Euscaphis konishii and other closely related species.
- 相关文献
作者其他论文 更多>>
-
Contrasting roles of landscape compositions on shaping functional traits of arthropod community in subtropical vegetable fields
作者：Zhang, Jie;Saqib, Hafiz Sohaib Ahmed;Niu, Dongsheng;Guaman, Karla Giovana Gavilanez;Wang, Ao;Yu, Deyi;You, Minsheng;Pozsgai, Gabor;You, Shijun;Zhang, Jie;Yu, Deyi;Zhang, Jie;Niu, Dongsheng;Wang, Ao;You, Minsheng;Saqib, Hafiz Sohaib Ahmed;Niu, Dongsheng;You, Minsheng;Pozsgai, Gabor;You, Shijun
关键词：Habitat transformation; Biological pest control; Functional diversity; Trait composition; Scale-dependent; RLQ analysis
-
A mixture of p-anisaldehyde and ethyl nicotinate elicits positive antennal and behavioral responses in Frankliniella occidentalis
作者：Tian, HouJun;Chen, YiXin;Chen, Yong;Chen, XiuQin;Lin, Shuo;Wei, Hui;Tian, HouJun;Chen, YiXin;Chen, Yong;Chen, XiuQin;Lin, Shuo;Wei, Hui;Tian, HouJun;Chen, YiXin;Chen, Yong;Chen, XiuQin;Lin, Shuo;Wei, Hui;Tian, HouJun;Chen, YiXin;Chen, Yong;Lin, Shuo;Wei, Hui;Tian, HouJun;Chen, YiXin;Chen, Yong;Lin, Shuo;Wei, Hui;Zhang, Jie;Yang, Guang;Yang, Guang
关键词：antennal and behavioral responses; EAG; electroantennography; ethyl nicotinate; greenhouse trapping; lure; mixture; p-anisaldehyde; Thripidae; Thysanoptera; western flower thrips; Y-tube olfactometer
-
ABA and Bud Dormancy in Perennials: Current Knowledge and Future Perspective
作者：Pan, Wenqiang;Liang, Jiahui;Li, Jingru;Liu, Chang;Xin, Yin;Zhang, Yanmin;Wang, Shaokun;Zhao, Yajie;Zhang, Jie;Yi, Mingfang;Wu, Jian;Sui, Juanjuan;Zhang, Jie;Gazzarrini, Sonia;Gazzarrini, Sonia
关键词：ABA; perennials; bud dormancy; hormone; sucrose; epigenetics
-
Tripartite interactions between jasmonic/salicylic acid pathways, western flower thrips, and thrips-transmitted tomato zonate spot virus infection in Capsicuum annuum
作者：Zhao, Lihua;Chen, Yongdui;Zheng, Kuanyu;Zhang, Jie;Chen, Yong;Mu, Ye;Zhao, Xingyue;Gao, Yulin;Zheng, Limin
关键词：Antiviral defense; Host plant resistance; Plant hormones; Tospovirus; Vector fitness
-
A plant virus mediates interspecific competition between its insect vectors inCapsicuum annuum
作者：Chen, Yong;Wei, Hui;Zheng, Xue;Chen, Yong-Dui;Zheng, Kuan-Yu;Zhao, Li-Hua;Zhang, Jie;Mu, Ye;Zhao, Xing-Yue;Gao, Yu-Lin;Zheng, Li-Min
关键词：Tomato zonate spot virus; Frankliniella occidentalis; Thrips palmi; Interspecific competition; Reproductive competition
-
Comparative analysis of genetic polymorphisms among Monascus strains by ISSR and RAPD markers
作者：Yang, Cheng-long;Huang, Ying-ying;Wu, Xiao-ping;Chen, Bingzhi;Chen, Zhang-e;Jin, Shan-shan;Deng, Si-shan
关键词：Monascus;genetic diversity;cluster analysis;molecular marker
-
Appraisal of the impact of three insecticides on the principal rice pests and their predators in China
作者：Chen, Yong;Wei, Hui;Chen, Yong;Wei, Hui;Zheng, Xue;Chen, Yongdui;Su, Xiaoxia;Zhang, Jie;Liu, Jie
关键词：insecticide;planthopper;rice leaffolder;predator;community
