Structural characterization and immune enhancement activity of a novel polysaccharide from Moringa oleifera leaves
文献类型： 外文期刊
作者： Li, Chao 1 ; Dong, Zhuping 1 ; Zhang, Bin 1 ; Huang, Qiang 1 ; Liu, Guang 2 ; Fu, Xiong 1 ;
作者机构： 1.South China Univ Technol, Natl Joint Res Ctr Trop Hlth Foods, Sch Food Sci & Engn, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Guangzhou 510640, Peoples R China
2.Guangdong Acad Agr Sci, Minist Agr & Rural Affairs, Guangdong Key Lab Agr Prod Proc, Key Lab Funct Foods,Sericultural & Agrifood Res I, Guangzhou, Peoples R China
3.Overseas Expertise Intro Ctr Discipline Innovat F, Ctr 111, Guangzhou, Peoples R China
关键词： Moringa oleifera; Polysaccharides; Linkage analysis; Immunoactivity; Macrophages
期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）
ISSN： 0144-8617
年卷期： 2020 年 234 卷
页码：
收录情况： SCI
摘要： A novel polysaccharide, designated as MOP-3, was extracted and isolated from the leaves of Moringa oleifera. Structural characterization showed that MOP-3 had a molecular weight (MW) of 4.033 x 10(6) Da and was composed of arabinose, glucose and galactose with a molar ratio of 47.73:1.00:57.65. Methylation and nuclear magnetic resonance (NMR) analysis revealed that the main glycosidic linkage types of MOP-3 were (1 -> 3,6)-beta-D-Galp, (1 -> 6)-beta-D-Galp, (1 -> 5)-alpha-L-Araf, and T-alpha-L-Araf. MOP-3 containing no lipopolysaccharides showed no direct cytotoxicity to the macrophage RAW 264.7 cells within the tested concentrations. The immunomodulatory assay suggested that MOP-3 could significantly enhance pinocytic capacity and increase the secretion of reactive oxygen species (ROS), nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) by up-regulating the corresponding mRNA expression levels in RAW 264.7 cells. Our findings demonstrated that MOP-3 could potentially be used as a functional food for the hypoimmunity therapies.
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