您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of odorant-binding proteins and functional analysis of antenna-specific AplaOBP1 in the emerald ash borer, Agrilus planipennis

文献类型： 外文期刊

作者： Wang, Shan-Ning 1 ; Shan, Shuang 2 ; Yu, Guo-Yue 1 ; Wang, He 3 ; Dhiloo, Khalid Hussain 5 ; Khashaveh, Adel 2 ; Zhang 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing Key Lab Environm Friendly Management Frui, Beijing 100097, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Beijing Forestry Protect Stn, Beijing 100029, Peoples R China

4.China Agr Univ, Coll Plant Protect, Beijing 100193, Peoples R China

5.Sindh Agr Univ Tandojam, Fac Crop Protect, Dept Entomol, Hyderabad 70060, Pakistan

关键词： Agrilus planipennis; Odorant-binding proteins; Sensilla basiconica; Fluorescence binding assay; Electroantennography

期刊名称：JOURNAL OF PEST SCIENCE （ 影响因子：5.918； 五年影响因子：5.955 ）

ISSN： 1612-4758

年卷期： 2020 年 93 卷 2 期

页码：

收录情况： SCI

摘要： Olfaction-based strategies have been successfully applied to monitor the emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae); however, roles of chemosensory-associated proteins in olfactory perception of A. planipennis are rarely reported. Here, we identified 11 odorant-binding proteins (OBPs) from the A. planipennis transcriptome and genome. Sequence and phylogenetic analyses revealed that A. planipennis OBPs (AplaOBPs) could be classified into four subfamilies. The reverse transcription PCR (RT-PCR) analysis showed that all three members of the ABPII, one plus-C OBP, and one minus-C OBP were specifically or predominantly expressed in the antennae of both sexes, indicating their possible involvement in chemoreception. Subsequently, an antenna-specific OBP, AplaOBP1, was investigated through in situ hybridization, immunocytochemistry, and fluorescent competitive binding assays. It was found that the AplaOBP1 gene was detected under the base of the antennal sensilla, and the AplaOBP1 protein was expressed in the lymph of multiparous sensilla basiconica types I and III. In vitro, recombinant AplaOBP1 exhibited high binding affinities (K-i = 3.38-9.25 mu M) with nine volatiles including five host-tree terpenes (e.g., myrcene, limonene, nerolidol, alpha-farnesene, and ocimene), which indicated that AplaOBP1 might be involved in the detection of host volatiles. The electrophysiological activities of all AplaOBP1 ligands were further confirmed using electroantennography, whose results imply that these volatiles may act as putative semiochemicals for A. planipennis. In conclusion, our results provide valuable insights into the molecular basis of olfaction in A. planipennis and help us use OBPs as targets to design novel olfactory regulators for management of A. planipennis.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台