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Effects of oregano essential oil on in vitro ruminal fermentation, methane production, and ruminal microbial community

文献类型： 外文期刊

作者： Zhou, Rui 1 ; Wu, Jianping 1 ; Lang, Xia 2 ; Liu, Lishan 2 ; Casper, David P. 3 ; Wang, Cailian 2 ; Zhang, Liping 1 ; Wei 1 ;

作者机构： 1.Gansu Agr Univ, Coll Anim Sci & Technol, 1 Yingmen Village Anning, Lanzhou 730070, Gansu, Peoples R China

2.Gansu Acad Agr Sci, Anim Husb Pasture & Green Agr Inst, 1 Nongkeyuan Village Anning, Lanzhou 730030, Gansu, Peoples R China

3.Caspers Calf Ranch, 4890 West Lily Creek Rd, Freeport, IL 61032 USA

关键词： bacterial composition; methane production; oregano essential oil; rumen fermentation; 16S rRNA

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2020 年 103 卷 3 期

页码：

收录情况： SCI

摘要： Different inclusion rates of oregano essential oil (OEO) were investigated for their effects on ruminal in vitro fermentation parameters, total gas, methane production, and bacterial communities. Treatments were (1) control, 0 mg/L of OEO (CON); 13 mg/L (OEO1); 52 mg/L (OEO2); 91 mg/L (OEO3); and 130 mg/L (OEO4), each incubated with 150 mL of buffered rumen fluid and 1,200 mg of substrate for 24 h using the Ankom in vitro gas production system (Ankom Technology Corp., Fairport, NY). Treatment responses were statistically analyzed using polynomial contrasts. Digestibility of DM, NDF, and ADF increased quadratically with increasing OEO inclusion rates. Digestibility of DM and NDF were highest for OEO2, whereas ADF digestibility was highest for OEO3, compared with CON, with the remaining treatments being intermediate and similar. Ammonia nitrogen concentrations decreased from CON at a quadratic rate with increasing OEO inclusion rates, and OEO2 had the lowest concentration compared with the other groups. Total VFA, acetate, propionate, butyrate, valerate, and isovalerate concentrations linearly decreased with increasing OEO inclusion rates. Total gas production levels by CON and OEO4 were greater than those of OEO1, OEO2, and OEO3 in a quadratic response, and methane production linearly decreased from CON, compared with OEO4, at a decreasing rate with OEO inclusion rates. As determined by 16S rRNA sequencing, the alpha biodiversity of ruminal bacteria was similar among OEO inclusion rates. Increasing OEO inclusion rates linearly increased the relative abundance of Prevotella and Lialister bacteria. Several bacteria demonstrated different polynomial responses, whereas several bacteria were similar among increasing OEO inclusion rates. These results suggested that OEO supplementation can modify ruminal fermentation to alter VFA concentrations and reduce methane emissions by extensively altering the rurninal bacterial community, suggesting an optimal feeding rate for future animal studies of approximately 52 mg/L for mature ruminants.

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